抗水牛伊氏锥虫变异表面糖蛋白抗原单克隆抗体的研制  

Preparation of Monoclonal Antibody against Variant Surface Glucoprotein Antigen of Trypanosoma evansi

作　　者：曾晓飞[1] 陈汉忠[1] 韦英益[1] 何木荣[1] 李晓栩[1] 

机构地区：[1]广西大学动物科技学院,南宁530005

出　　处：《中国畜牧兽医》2009年第10期72-74,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金资助项目(30660140);广西教育厅科研项目(200620)

摘　　要：用纯化的伊氏锥虫变异表面糖蛋白(variant surface glucoprotein,VSG)免疫BALB/c小鼠,取免疫小鼠脾细胞与SP2/0骨髓瘤细胞进行融合,经过3次克隆和间接ELISA方法筛选,获得3D7、5B9 2株稳定分泌单克隆抗体的杂交瘤细胞株,用间接ELISA方法检测杂交瘤细胞培养液上清效价和小鼠腹水效价,其中细胞培养上清效价分别为1∶6400和1∶12800,腹水效价分别为1∶105和1∶106。单抗的亚型鉴定结果表明,3D7、5B9分泌的抗体都为IgG1亚类κ链。The BALB/c mice were immunized with variant surface glucoprotein of Trypanosoma evansi.The spleen lymph cells were fused with mouse SP2/0 myeloma cells,two hybridoma cell lines 3D7 and 5B9 secreting monoclonal antibodies against variant surface glucoprotein were determined by indirect ELISA.Their titers in cell culture supernatant were 1∶6400 and 1∶12800,and titers of ascites were 1∶105 and 1∶106,both 3D7 and 5B9 belongs to IgG1 with κ chain.

关 键 词：变异表面糖蛋白 伊氏锥虫 单克隆抗体 

分 类 号：S859.79[农业科学—临床兽医学]