钙离子浓度对绵羊精子获能的影响  

Effects of Calcium Ion Concentration on Ovine Sperm Capacitation

作　　者：李贺娟[1] 倪和民[1] 薛君忠[1] 张宏[1] 杨飞鸽[1] 刘烈琴[2] 

机构地区：[1]北京农学院动物科学技术系,北京102206 [2]中国科学院动物研究所,北京100101

出　　处：《中国畜牧兽医》2009年第10期99-101,共3页China Animal Husbandry & Veterinary Medicine

基　　金：北京市教育委员会科技发展计划重点项目(KZ200510020013);2007年度教育部留学人员科技活动择优资助项目(启动类)部分资助

摘　　要：为探讨钙离子浓度对绵羊精子获能的影响,将绵羊精子在不同钙离子浓度的绵羊输卵管合成液(SOF液)中培养,并用钙离子载体A23187进行诱导。结果发现,不同钙离子浓度对绵羊精子的影响差别不明显,经A23187诱导后,1.7mmol/L组和2 mmol/L组的顶体反应率(AR)与其他组有显著差异(P<0.05),1.7 mmol/L浓度中,绵羊精子存活时间为16 h,2 mmol/L浓度中的存活时间为10 h,因此认为绵羊精子获能的适宜钙离子浓度为1.7 mmol/L,且获能过程中钙离子的存在是必要的。To discuss the effects of calcium ion concentration on ovine sperm capacitation. The sheep spermatozoa were in- cubated in the ovine oviduct synthesis fluid with different calcium ion concentration, then induced by calcium ionophore A23187. The results showed that the sperm capacitation was not significantly affected by adding with different concentrations of calcium ion, but the acrosome reaction rates induced by 1.7, 2 mmol/L were significantly higher than the others. The appropriate sperm enduring period of 1.7, 2 mmol/L were 16 h and 10 h respectively. It suggested that the suitable concentration of calcium ion for ovine sperm capacitation in vitro is 1.7 mmol/L, and this process should accompany with calcium ion.

关 键 词：绵羊 精子 钙离子浓度 获能 

分 类 号：Q813[生物学—生物工程]