^(137)Cs γ射线辐照对铁皮石斛种胚原球茎的诱变研究  

STUDY MUTAGESIS OF Dendrobium officinale PROTOCORM FROM EMBRYO IRRADIATED BY ^(137)Cs γ-RAYS

作　　者：詹忠根[1] 徐程[2] 席玙芳[3] 

机构地区：[1]浙江经贸职业技术学院,浙江杭州310018 [2]浙江大学生命科学学院,浙江杭州310012 [3]浙江大学生物系统工程与食品科学学院,浙江杭州310028

出　　处：《核农学报》2009年第5期816-819,共4页Journal of Nuclear Agricultural Sciences

基　　金：浙江省教育厅(Y200804108)资助项目

摘　　要：研究了不同剂量的137Csγ射线辐照对铁皮石斛种胚原球茎生长和分化效应的影响。结果表明:辐照处理对种胚原球茎的生长和分化有抑制作用,尤其是对根的抑制更为明显;由于种胚原球茎的完整性好,耐受性强,其辐照半致死剂量(LD50)为67.23Gy;流式细胞分析表明,大多数外部形态发生改变的植株其细胞内DNA的倍性发生了改变,出现相当多的非整倍体;此外,辐照产生的变异苗出现茎分叉、叶片缺绿或白绿相间等现象,可能是由于某些基因的表达与关闭引起的。Study on the growth and differentiation of D. officinale protocorm treated by different doses of 137Cs γ-ray was carried out. The results indicated that the treatment of irradiation restrained the growth and differentiation of protocorm, especially to roots.The LD50 is 67.23 Gy, because the protocorm is a integrate embryoids and higher tolerance was found. Ploidy analysis showed that most of aberrant seedlings go with DNA change, and they were not euploid. When the plants treated by irradiation, some of aberrant seedlings appeared stem forfication, and leaves appeared chlorophyll-deficient or green-white,probably due to some genes＇ expression or closing.

关 键 词：铁皮石斛 种胚原球茎 辐照诱变 

分 类 号：S567.239[农业科学—中草药栽培] O484[农业科学—作物学]