HBsAg阴性献血者乙肝五项标志物及HBV-DNA检测性分析  

Correlation analysis between 5 markers of hepatitis B and HBV-DNA among blood donors with HBsAg negative

作　　者：曹瑞华[1] 

机构地区：[1]阜阳市第二人民医院,安徽阜阳236015

出　　处：《中华疾病控制杂志》2009年第5期538-540,共3页Chinese Journal of Disease Control & Prevention

摘　　要：目的通过HBsAg阴性献血者乙型肝炎五项标志物和HBV-DNA的相关性分析,论证HBsAg阴性血液的安全性。方法应用酶联免疫法检测乙型肝炎五项标志物;应用实时荧光定量PCR检测技术检测HBV-DNA。结果3398例HBsAg阴性献血样本,HBV-DNA检测总阳性率为2.73%,其中HBsAb(+)、HBcAb(+)模式组占6.59%,HBV-DNA阳性率为5.80%;HBsAb(+)、HBeAb(+)、HBcAb(+)模式组占4.74%,HBV-DNA阳性率为6.83%;HBcAb(+)组占6.24%,HBV-DNA阳性率为5.19%;HBeAb(+)、HBcAb(+)模式组占5.86%,HBV-DNA阳性率为4.52%;HBsAb(+)模式组占31.99%,HBV-DNA阳性率为1.56%;全阴模式组占44.59%,HBV-DNA阳性率为2.11%。结论HBsAg阴性的献血者仍然存在HBV感染的危险,特别当单独HB-cAb检测阳性或合并HBsAb、HBeAb等阳性标志物时,输血潜在感染的风险增加,应同时检测乙肝其他标志物,推行灵敏度高的核酸扩增技术筛查血液,可以进一步减少输血感染率。Objective To discuss the security of negative HBsAg blood by correlation analysis between 5 markers of hepatitis B and HBV-DNA among blood donors with HBsAg negative. Methods 5 markers of hepatitis B were detected by ELISA and HBV-DNA by real-time PCR. Results In 3 398 samples of negative HBsAg blood donors, the total positive rate of HBV-DNA was 2.73 %. Among them, the type series of HBsAb（ ＋ ）, HBcAb（ ＋ ） account 6.59%, the positive rate of HBV-DNA was 5.80% ; the type series of HBsAb（ ＋ ）, HBeAb（ ＋ ）, HBcAb（ ＋ ）account 4.74% , the positive rate of HBV- DNA was 6.83 % ; the type series of HBcAb（ ＋ ） alone account 6.24 %, the positive rate of HBV-DNA was 5.19% ; the type series of HBeAb（ ＋ ）, HBcAb（ ＋ ） account 5.86% , the positive rate of HBV- DNA was 4.52% ; tee type series of HBsAb（ ＋ ） alone account 31.99% , the positive rate of HBV- DNA was 1.56 % ; the type series of entire negative results account 44.59 %, the positive rate of HBV-DNA was 2.11%. Conclusions Blood donors with negative HBsAg still have the risk of infecting HBV, especially when HBcAb is positive alone, or combining with other positive markers such as HB-sAb, HBeAb, latent transfusion＇infection, the risk was remarkably increased. As a result, we should detect the other markers of hepatitis B simultaneously and carry out real-time PCR technique to screen blood in order to reduce infection rate arising from transfusion.

关 键 词：献血者 输血 肝炎 乙型 慢性 DNA HBV 

分 类 号：R512.62[医药卫生—内科学]