酒精废液降解菌Q5的化学诱变  

Study on Alcohol Wastewater Degradation Bacterial Q5 by Chemical Induced Mutations

作　　者：周盛[1] 

机构地区：[1]广西玉林师范学院化生系,广西玉林537000

出　　处：《生物技术》2009年第5期52-54,共3页Biotechnology

基　　金：广西教育厅科研项目(200608LX109)资助

摘　　要：目的:筛选出一些酒精废液的降解菌。方法:使用亚硝基胍(NTG)、亚硝基甲基脲(NMU)等对酒精废液降解菌Q5进行诱变。结果:在无机盐选择培养基上生长菌株有36株,再经5次传代试验仅有8株能稳定传代,最后筛选出以酒精废液为碳源的性能较好的突变菌株Q58。该突变菌株Q58对酒精废液的降解率比出发菌株高0.65%;对酒精废液的降解时间比出发菌株缩短12h。Objective：Some alcohol wastewater degradation bacterial were screened.Method：Q5 was induced by NTG and NMU.Result：Thirty six target strains had been got on the selective culture medium,in which eight strains of them have stable heredity through five times heredity experiments.Mutation Q58 was obtained for its better degradation function.Q58 can use alcohol wastewater as only carbon.Compared with the original Q5,mutation Q58 could improve alcohol wastewater degradation rate 0.65% and the degradation time can be shorten 12 hours.

关 键 词：酒精废液 诱变育种 降解时间 

分 类 号：Q789[生物学—分子生物学]