一个编码锌指蛋白的大豆疫霉基因Ps-zfA1的克隆与转录分析  

Cloning and Transcription of Ps-zfA1,an Encoding Putative Zn-finger Protein Gene from Phytophthora sojae

作　　者：王子迎[1] 王朝霞[1] 沈洁[1] 

机构地区：[1]合肥师范学院生命科学系,安徽合肥230061

出　　处：《激光生物学报》2009年第4期504-508,449,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30800040);安徽省高校优秀青年人才项目(2007jqL105)

摘　　要：对大豆疫霉野生菌株与紫外线诱导的卵孢子缺失突变株进行差异表达基因分析,筛选到一个在大豆疫霉卵孢子形成过程中特异表达、编码CCHC型锌指蛋白的cDNA片段。克隆了该基因的全长序列,命名为Ps-zfA1。Southern杂交结果显示,Ps-zfA1在大豆疫霉基因组中只有2个拷贝。系统发育分析表明,Ps-zfA1与三角褐指藻Phaeodactylum tricornutum的锌指蛋白的序列同源性最高,且该基因编码的氨基酸序列具有一个CCHC型锌指蛋白典型的保守结构域。时实定量RT-PCR分析表明,该基因在大豆疫霉卵孢子形成过程中特异表达,且表达量随着产孢培养的时间延长而升高。Using the method of suppression subtractive hybridization （ SSH）, a Phytophthora sojae cDNA coding Znfinger protein was identified, named Ps-zfA1, which was up-regulated during the early stage of oosporogenesis. Southern blot shows that there are maybe two copies of Ps-zfA1 in the P. sojae genome. The predicted amino acid sequences of Ps- zfA1 embody a CCHC motif and shows very close relationship with Phaeodactylum tricornutum in phylogenic tree. Quantitative real-time RT-PCR analysis shows that the transcript of the Ps-zfA1 was not found in the uhraviolet mutation, it was found only in wild strain, and the transcriptional level of Ps-zfA1 was up-regulated with days during oosporogenesis.

关 键 词：大豆疫霉 锌指蛋白 卵孢子发育 紫外诱变 

分 类 号：Q939.96[生物学—微生物学]