细鳞斜颌鲴不同组织中LDH同工酶的比较研究  

A Study on LDH Isozymes in Different Tissues of Plagiognathops Microlepis

作　　者：杨品红[1,2,3] 徐黎明 王志陶[1] 万美娇[1] 覃忠元 张倩 陈鸿星 

机构地区：[1]湖南文理学院生命科学学院,湖南常德415000 [2]湖南省水产工程技术研究中心,湖南常德415000 [3]大湖水殖股份有限公司,湖南常德415000

出　　处：《激光生物学报》2009年第4期509-515,共7页Acta Laser Biology Sinica

基　　金：国家重点星火计划(2003EA770038);湖南省重点攻关项目(01NKY2004-1);湖南省"十一五"重点建设学科"动物学"项目(07-A-2);湖南省高校产学研究合作示范基地--水生生物资源与利用资助

摘　　要：采用垂直板聚丙烯酰胺凝胶电泳技术和特异性染色方法分析了细鳞斜颌鲴脑、肾脏、肝脏、肌肉、心脏和眼6种组织的LDH同工酶,结果表明酶谱的表达具有明显的组织特异性。酶带共由三个基因座位(Ldh-a、-b-、c)编码,在6种组织中共检测到7条带,其中脑、肾脏、心脏和眼四种组织中均发现3条带,肝脏中7条带,肌肉中1条带且为超显性,在PAGE胶趋向阴极侧检测到4条肝脏组织所特有的Ldh-c基因编码带;各组织中酶带活性顺序为脑LDH-1>LDH-2>LDH-3,肾脏LDH-3>LDH-1>LDH-2,肝LDH-3>LDH-5>LDH-2>LDH-6>LDH-1>LDH-4>LDH-7,心脏LDH-1>LDH-2>LDH-3,眼LDH-3>LDH-1>LDH-2,肌肉仅有LDH-3;由迁移率R f(LDH-A4)<R f(LDH-B4)判断细鳞斜颌鲴LDH同工酶泳动类型为B>A;对酶谱进行扫描分析及亚基计算得到了一致的结果:脑和心脏两种组织中Ldh-b基因表达占优势而肾脏、肝脏、眼和肌肉中Ldh-a基因表达占优势。Plagiognathops microlepis, which has a wide range of food resouse, humus, for example, organic debris and algal, is an important economical species and well-known as scavenger. By using vertical polyacrylamide gel electropho- resis and specific dye measures, LDH isozymes in 6 tissues （ brain, kidney,liver, heart, muscle and eyes） was investigated. The results showed that the electrophoretograms of LDH isoenzyme patterns exhibited remarkable tissue-specifici- ties： 7 LDH isoenzyme bands, encoded by Ldh-α, Ldh-b and Ldh-c three loci, were detected from all the 6 tissues, 3 bandes were found in brain, kidney, heart and eyes, while in liver and muscle the bands were 7 and 1 , respectively, the only one band found in muscle was predominant in expressing, 4 specific bands encoded by Ldh-c were detected on the cathode side of PAGE hectograph withing liver. The sequence of isoenzyme activity in brain, kidney,liver,heart and eyes are LDH-1 〉 LDH-2 〉 LDH-3, LDH-3 〉 LDH-1 〉 LDH-2, LDH-3 〉 LDH-5 〉 LDH-2 〉 LDH-6 〉 LDH-1 〉 LDH-4 〉LDH-7, LDH-1 〉 LDH-2 〉 LDH-3 and LDH-3 〉 LDH-1 〉 LDH-2, respectively; while the isoenzyme activity of the only one band（ LDH-3 ） detected in muscle was 100% ; The rates of flow（ Rf ） for isoenzymes LDH-A4 was inferior than LDH- B4, indicating that the eleetrophoretic style of LDH isozymes in Plagiognathops microlepis is B 〉 A ; Consistent results were acquired by scanning and analyzing the sub unit in LDH isoenzymes, namely, Ldh-α was preponderantly in expressing in kidney,liver,muscle and eyes, While Ldh-b was preponderant in heart and brain.

关 键 词：细鳞斜颌鲴 LDH同工酶 聚丙烯酰胺凝胶电泳 组织特异性 

分 类 号：S917[农业科学—水产科学]