苹果砧木珠眉海棠盐胁迫应答基因的微列阵研究  

Microarray analysis of salt-stress responsive genes in Malus zumi Mats

作　　者：刘佳[1] 李擎天[1] 孔瑾[1] 许雪峰 韩振海[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国农业大学学报》2009年第5期61-67,共7页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(30600416);北京市自然科学基金资助项目(5072027)

摘　　要：以盐胁迫下苹果的耐盐砧木珠眉海棠为材料,用SMARTTM方法构建了cDNA文库。随机挑取5 000个cDNA克隆制备芯片,得到差异表达的基因388个,其中249个表达上调、139个表达下调。分析其中变化量在8倍以上的42个基因,并对部分基因进行RT-PCR验证,结果与芯片杂交结果一致。根据功能把这些基因分为5类:光合作用相关基因、物质转运相关基因、基础代谢相关基因、逆境相关基因以及其他基因。其中直接参与盐应答的基因占34%,包括上游调控蛋白、合成植体内小分子渗透调节物的限速酶、胁迫产生的活性氧清除剂、直接调节离子运输和储存的蛋白等。用此cDNA微列阵体系,将基因芯片技术与cDNA文库相结合,成为全面研究盐胁迫下基因表达的有效途径。为进一步研究盐应答基因功能及盐胁迫机制提供参考。A cDNA library was constructed in Malus zumi Mats under salt stress by SMARTTM . 5 000 cDNA clones were randomly selected for microarray analysis. 388 genes were identified responding to salt stress,among which 249 genes were up-regulated and 139 genes down-regulated. 42 genes,whose expression changed more than 8 folds under salt stress were sequenced. 4 genes selcted for RT-PCR analysis showed similar expression pattern with microarry. The 42 genes can be classified into 5 groups： photosynthesis-related genes, transport-related genes, metabolism-related genes, stress-related genes and the others genes. The results showed that salt stress influenced many aspects of plant growth in M. zumi Mats~ some of these genes may play important roles in plant salt tolerance. Our findings may result in further functional study of salt-response genes in M. zumi Mats and the elucidation of its molecular mechanism of response, to salt stress.

关 键 词：珠眉海棠 盐胁迫 CDNA文库 基因芯片 差异表达基因 

分 类 号：S611.1[农业科学—园艺学] S601