新城疫病毒HN基因主要抗原位点区段的原核表达  

Prokaryotic Expression of Antigen Sites of the Newcastle Disease Virus Hemagglutinin-Neuraminidase

作　　者：罗晶璐[1] 齐岩[1] 任涛[1] 

机构地区：[1]农业部养禽与禽病防治重点开放实验室,华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2009年第4期90-93,共4页Journal of South China Agricultural University

基　　金：863计划项目(2006AA10A205);教育部“长江学者和创新团队发展计划”创新团队项目(IRT0723);广东省自然科学基金(5500-E08020);广东省科技计划项目(2007A020100006)

摘　　要：利用生物软件对新城疫病毒GD/GM/03/Ch的血凝素神经氨酸酶（HN）蛋白进行抗原特性分析,选定169-267位氨基酸、318-405位氨基酸和448-571位氨基酸3个区域作为多肽表位候选区域.用含新城疫病毒HN基因的重组质粒为模板,设计引物通过PCR扩增获得HNa、HNb和HNc 3个抗原结构域基因片段,分别经双酶切定向克隆到原核表达载体pBT上进行表达,并构建了pBT-HNa-b-c串联重组质粒进行表达,经过SDS-PAGE和W est-ern-b lot分析,验证了表达产物具有反应原性.Antigenicity analysis of Newcastle disease virus （NDV） strain GD/GM/03/Ch hemagglutininneuraminidase（HN） protein was studied by using biological software, the selection of 169 -267AA,318 -405AA and 448 -571AA as candidates for peptide epitopes was determined. The gene fragments HNa, HNb and HNc coding for the antigenic structural domains of the NDV HN amplified from the recombinant plasmid containing full length NDV HN gene by regular PCR with specific primers. The genes were respectively inserted into pBT vector after cleavage by corresponding enzymes. The recombinant plasmids pBT-HNa-b-c were also constructed to carry out the expression. It was found that all the expression products were recognized by NDV positive serum in Western-blot test.

关 键 词：新城疫病毒 HN基因 抗原位点 原核表达 

分 类 号：S852.659.5[农业科学—基础兽医学]