狂犬病毒分离株N基因的分析  

Analysis of Nucleoprotein Genes of Isolated Strain of Rabies Virus

作　　者：江飙[1] 刘晓慧[1] 陈晶[1] 孙招金[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2009年第4期119-121,共3页Journal of South China Agricultural University

基　　金：国家自然科学基金(30671565);科技部微生物资源平台项目(2005DKA21205-9);广东省自然科学基金团队项目(5200638)

摘　　要：运用RT-PCR和动物接种的方法从广东省东莞市正常犬只的唾液中分离获得1株狂犬病毒,编号为GD33.为了解析该毒株与兽用狂犬病弱毒疫苗的关系,对该分离株的N基因进行了克隆和测序,并将N基因的序列与GeneBank上已发表的狂犬病毒N基因核苷酸和推导的氨基酸序列进行比较.结果发现:该分离株仅能引起乳鼠发病,对6周龄成鼠无致病性,说明该毒株不是强毒.该分离株N基因与弱毒疫苗HEP-F lury N基因的相似性最高,达99.7%,并且同处系统发育树的最近分枝.因此,推测该分离株源自接种的弱毒疫苗.A rabies virus strain designated GD33 was isolated and identified by mice inoculation and RTPCR. To analyze the relationship between the isolated strain and the attenuated rabies virus vaccine strain for domestic dog, the N gene of the isolated strain was cloned and sequenced. The sequences of nueleotide and the deduced amino acid of the N gene were compared with that of other rabies virus strains published. The results indicated that the isolated strain was an attenuated rabies virus because it killed only suckling mice and was apathogenic to six-week-old adult mice. The homology of N gene between GD33 strain and the attenuated HEP-Flury strain was 99.7% and both strains were on the same branch of phylogenetie tree. Therefore, they speculated that the isolated strain was originated from the attenuated vaccine strain.

关 键 词：狂犬病毒 弱毒疫苗 N基因 克隆 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]