黄菠萝缓慢生长法离体保存技术的研究  

Study on slow-growth conservation in vitro of Phellodendron amurense

作　　者：杨学成[1] 邹建军[2] 李绍臣[2] 李凤鸣[2] 张立民[2] 陈建军[2] 王培忠[3] 赵在立 

机构地区：[1]双辽市林业局,吉林四平136400 [2]吉林省林业科学研究院,吉林长春130033 [3]国家林业局驻长春专员办,吉林长春130022 [4]长春市林业科学研究院,吉林长春130117

出　　处：《吉林林业科技》2009年第5期15-17,38,共4页Journal of Jilin Forestry Science and Technology

摘　　要：缓慢生长法是植物种质资源保存切实可行的方法之一,目前已被广泛应用。本研究利用组织培养技术对黄菠萝缓慢生长法的适宜基本培养基和糖的质量浓度进行了筛选和研究,结果表明:适宜黄菠萝缓慢生长的组培体系是1/2MS+80 g.L-1蔗糖。为成功离体保存黄菠萝种质资源奠定了基础。The slow-growth is one of the effective methods of plant germplasm resource conservation,which is practical and widely used now.In this study,the basic culture medium and sugar concentration for slow-growth of Phellodendron amurense were screened by tissue culture technique.The results showed that the suitable tissue culture system was 1/2MS＋80 g·L^-1 sucrose,which providing the foundation for in vitro conservation of germplasm resource of Phellodendron amurense.

关 键 词：黄菠萝 缓慢生长法 离体保存 种质资源 

分 类 号：S668.4[农业科学—果树学] TN304.12[农业科学—园艺学]