不同配比头孢呋辛钠/舒巴坦钠的体外药效学研究  

In vitro antibacterial activities of cefuroxime/sulbactam in different compositions

作　　者：王跃明[1] 赵昆[1] 李聪然[1] 杨信怡[1] 向倩[1] 娄人慧[1] 刘京芳[1] 张伟新[1] 陈慧贞[1] 游雪甫[1] 

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所,北京100050

出　　处：《中国抗生素杂志》2009年第10期621-631,共11页Chinese Journal of Antibiotics

基　　金：国家十一五科技重大专项资助项目(2009ZX09303-005;2008ZX09305-001);国家自然科学基金资助项目(30672502);北京自然科学基金资助项目(7062064)

摘　　要：目的评价头孢呋辛钠／舒巴坦钠（cefuroxime／sulbactam，CXM／SB）的体外抗菌活性。方法采用平皿二倍稀释法测定CXM／SB对1436株临床分离菌的MIC分布、试管二倍稀释测定最低杀菌浓度、活菌落计数法测定杀菌曲线。结果CXM／SB1：1和CXM／SB2：1对1436株临床分离菌具有广谱抗菌作用，抗菌活性与哌拉西林／三唑巴坦、头孢哌酮／舒巴坦相近，强于CXM／SB3：1、4：1、8：1。CXM／SB1：1和CXM／SB2：1对1109株产β-内酰胺酶菌株的MIC90值比CXM低4～32倍，对产ESBLs大肠埃希菌、肺炎克雷伯菌的MIC90值比CXM低16和8倍。不同pH值、细菌接种量、血清浓度对CXM／SB2：1抗金葡菌、大肠埃希菌、肺炎克雷伯菌的活性无明显影响。结论sB增强了CXM的抗菌活性，CXM／SB1：1和CXM／SB2：1具有较强和相近的广谱抗菌活性。Objective To investigate the in vitro antibacterial activities of cefuroxime/sulbactam （CXM/SB） in different compositions. Methods The in vitro antibacterial activity of CXM/SB against clinical isolated bacteria was determined by agar dilution method. The minimal bactericidal concentrations were determined by two-fold broth dilution method. The bacterial time-killing curves were acquired by viable bacteria count. Results The CXM/SB 1：1 and 2： 1 displayed potent and broad-spectrum antibacterial activities against 1436 stains of clinical isolated bacteria, which were similar to piperacillin/tazobactam and cefoperazone/sulbactam and better than CXM/SB 3： 1, 4： 1 and 8： 1. MIC90 of CXM/SB 1：1 and 2：1 was lower than CXM by 4 -32 folds against 1109 stains of 13-1actamases producing bacteria, and was lower by 16 folds and 8 folds against ESBLs-producing E. coli and K. pneumoniae respectively. The inoculum size, pH and serum concentration have no apparent effect on the inhibitory activity of CXM/SB 2：1 against S. aureus, E. coli and K. pneumoniae. Conclusion SB enhanced antibacterial activity of CXM. CXM/SB 1：1 and 2：1 have the similar pharmacodynamic functions with potent and broad-spectrum antibacterial activity.

关 键 词：头孢呋辛 舒巴坦 最低抑菌浓度 体外抗菌活性 

分 类 号：R978.11[医药卫生—药品]