拔节至成熟期遮荫对小麦籽粒高分子量麦谷蛋白亚基积累及GMP含量的影响  

Effects of Shading From Jointing Stage to Maturity Stage on High Molecular Weight Glutenin Subunits Accumulation and Glutenin Macropolymer Content in Wheat Grain

作　　者：伯云[1] 李华伟[1] 牟会荣[1] 蔡剑[1] 周琴[1] 戴廷波[1] 曹卫星[1] 姜东[1] 

机构地区：[1]南京农业大学农学院/农业部南方作物生理生态重点开放实验室/江苏省信息农业高技术研究重点实验室,南京210095

出　　处：《中国农业科学》2009年第10期3451-3458,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30671216;30700483);江苏省自然科学基金(BK2008329);教育部新世纪优秀人才资助计划(06-0493);现代农业产业技术体系项目

摘　　要：【目的】高分子量麦谷蛋白亚基(HMW-GS)和麦谷蛋白大聚合体(GMP)含量是反映小麦籽粒品质的重要性状,本试验研究拔节到成熟期遮荫对2个含相同HMW-GS亚基类型(7+8、2+12)小麦品种胚乳HMW-GS积累动态和GMP含量的影响。【方法】采用SDS-PAGE电泳和切胶比色的方法,对遮荫条件下小麦籽粒中HMW-GS亚基进行定量分析。【结果】遮荫使HMW-GS的起始形成时间提早,18%和25%重度遮荫处理缩短各亚基和总亚基快速积累期,并降低灌浆后期积累速度,成熟期单粒总HMW-GS积累量低于对照,但成熟期籽粒HMW-GS和GMP含量高于对照。轻度遮荫(10%)延长了籽粒HMW-GS快速积累期,亚基积累量和含量均高于对照。【结论】遮荫处理影响小麦籽粒HMW-GS积累,但其效应与遮荫强度有关。【Objective】 Accumulations of high molecular weight glutenin subunits （HMW-GS） and glutenin macropolymer （GMP） play key roles in determining grain quality in wheat （Triticum aestivum L.）. Two wheat cultivars owning the same HMW-GS type （7＋8 and 2＋12） were selected to study the effects of shading from jointing to maturity on HMW-GS accumulation and GMP content in grain. 【Method】 HMW-GS was firstly separated using the sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE）,and each HMW-GS subunit band in the gel was cut and extracted. Content of each HMW-GS subunit was then quantified by colorimetric analysis of the HMW-GS subunit extraction. 【Result】 Shading from jointing and maturity promoted the initial formation time of HMW-GS. The severe shading treatments （18% and 25%） shortened the rapid accumulation period of HMW-GS and reduced the accumulating rate of total HMW-GS during late grain filling period. Thus,the accumulation amount of total HMW-GS at maturity was lower under these treatments than the non-shading treatment. However,contents of HMW-GS and GMP were higher under severe shading than the control. A low-degree shading treatment （10%） prolonged the rapid accumulation period of HMW-GS,and increased the final accumulation amount and content of total HMW-GSP. 【Conclusion】 Shading from jointing to maturity obviously affected the accumulation amounts and contents of total HMW-GS in wheat grain. However,this regulatory effect was related to shading intensity.

关 键 词：小麦 遮荫 高分子量谷蛋白亚基(HMW-GS) 谷蛋白大聚合体(GMP) 

分 类 号：S512.1[农业科学—作物学] S512.101