促黄体素对绵羊卵母细胞体外成熟核质同步化的影响  

Effect of LH on Nuclear and Cytoplasm Synchronization for in vitro Maturation of Ovine Oocytes

作　　者：李凯[1] 倪和民[1] 赵雁伟[1] 刘云海[1] 曲杨燕[1] 李晓燕[1] 郭勇[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《中国农业科学》2009年第10期3633-3638,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30871836);北京市教育委员会科技发展计划重点项目(KZ200510020013)

摘　　要：【目的】研究促黄体素(LH)对绵羊卵母细胞体外成熟和体外受精的影响。【方法】将绵羊卵母细胞置于含LH(处理组)或不含LH(对照组)的成熟液中进行体外成熟,在不同时间点取出,观察卵母细胞核成熟情况;结合皮质颗粒分布变化的观察结果判断细胞质成熟情况。【结果】绵羊卵母细胞体外成熟4h时,处理组生发泡破裂(GVBD)率显著低于对照组(36.76%vs50%,P<0.05);体外成熟24h并进行体外受精后,处理组卵母细胞卵裂率和囊胚率显著高于对照组(67.15%vs42.37%,21.9%vs12.71%,P<0.05)。激光共聚焦扫描检测结果表明,随着核的成熟,绵羊卵母细胞的皮质颗粒由胞质中心区域向周边发生规律性迁移,经LH处理的卵母细胞,皮质颗粒扩散的程度明显好于对照组。【结论】LH可能通过延迟生发泡破裂和促进胞质成熟的方式,使绵羊卵母细胞的核质成熟趋于同步,有助于绵羊卵母细胞的体外成熟以及受精后的进一步发育。【Objective】 This study was made to investigate the effect of LH on maturation and fertilization in vitro of ovine oocytes. 【Method】 The ovine oocytes were matured in vitro with or without LH,and checked by confocal laser scanning microscope to observe the distribution of cortical granules during different durations stained with FITC-LCA. Meanwhile,some in vitro matured oocytes were also fertilized in vitro for analysis of their developmental potentiality further. 【Result】 After 4 h of culture,there were significant differences in the rate of GVBD between the treatment （with LH） and control （without LH） groups （36.76% vs 50%,P〈0.05）,moreover,there were also significant differences in cleavage rate and blastocyst rate,respectively,between these two groups （67.15% vs 42.37%,21.9% vs 12.71%,P〈0.05）. The distribution of cortical granules appeared to spread from the edges to the central site of sheep oocytes following their in vitro maturation durations. 【Conclusion】 LH plaies a role to delay the occurrence of germinal vesicle,prolong the maturation duration of cytoplasm,enhance the nuclear and cytoplasm synchronization of ovine oocytes matured in vitro,and finally improve the potentiality of their in vitro development.

关 键 词：绵羊 卵母细胞 体外成熟 促黄体素 核质同步成熟 

分 类 号：S826[农业科学—畜牧学]