PPARγ基因5'侧翼区单倍型与鸡生长和体组成性状的相关研究  

Correlation Between Peroxisome Proliferator-Activated Receptor γ 5'Flanking-Region Haplotypes with the Growth and Body Composition Traits in Chickens

作　　者：韩青[1] 王守志[1] 户国[1] 李辉[1] 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030

出　　处：《中国农业科学》2009年第10期3647-3654,共8页Scientia Agricultura Sinica

基　　金：国家"863"项目(2006AA10A120);黑龙江省自然科学基金重点项目(ZJN0604-01);国家"973"项目课题(2006CB102105)

摘　　要：【目的】研究PPARγ基因5'侧翼区多态性及其单倍型效应对鸡生长和体组成性状的影响,寻找影响肉鸡生长和体组成性状的QTL,为分子标记辅助选择提供相应依据。【方法】以东北农业大学肉鸡高、低腹脂双向选择品系第8、9和10世代肉仔鸡为试验材料,采用测序、PCR-RFLP等方法进行多态性位点检测及基因型分析。利用基因型信息重构单倍型并分析不同单倍型与生长和体组成性状的关系。【结果】PPARγ基因5'侧翼区存在3个多态性位点(g.-1784_-1768del17,c.-1241G>A及c.-75G>A),利用其构建的单倍型对腹脂重、腹脂率、肝脏重、肝脏比例、胫骨长、股骨重、龙骨长、跖骨围等性状有显著影响(P<0.05);对胸大肌重、胸小肌重、胸小肌率、跖骨长等性状具有一定的影响(P<0.2)。最小二乘分析结果表明,对于腹脂重、腹脂率等性状,BGA单倍型个体显著高于其它单倍型个体(P<0.05);相对于其它单倍型个体,AAG单倍型个体有较高的肝脏重、肝脏比例、胸小肌重和胸小肌率(P<0.05);在股骨重、胫骨长、跖骨长、跖骨围、龙骨长等骨骼生长发育性状方面,AAG单倍型个体显著优于AGG单倍型个体(P<0.05)。【结论】PPARγ基因5'侧翼区可能存在影响鸡脂肪性状的QTL,PPARγ基因可能是影响鸡骨骼性状的重要基因之一。【Objective】 The chicken PPARγ gene 5＇ flanking region was used to detect the polymorphisms,and the correlation between the polymorphism and growth and body composition traits were analyzed in order to find the quantitative trait locus which providing a basis for molecular marker-assisted selection. 【Method】The 8^th to 10^th generation populations of the Northeast Agricultural University broiler lines divergently selected for abdominal fat content were used. Growth and body composition traits were measured in the above populations. Polymorphisms among individuals were detected by DNA sequencing,PCR-RFLP and some other methods. The correlation analysis between the haplotypes constructed with the three polymorphisms detected and the growth and body composition traits was performed. 【Result】 The correlation analysis showed that haplotypes,based on three polymorphisms in 5＇ flanking region of PPARγ gene （g.-1784_-1768del17,c.-1241GA and c.-75GA）,were significantly correlated with abdominal fat weight （AFW）,abdominal fat percentage （AFP）,liver weight （LW）,liver weight percentage （LW/BW）,shank length （ShL）,femur weight （FeW）,feel length （KeL）,metatarsus circle （MeC） （P〈0.05）,and suggestively significantly correlated to some extents to pectoralis major weight （PMaW）,pectoralis minor weight （PMiW）,pectoralis minor weight percentage （PMiW/BW）,metatarsus length （MeL） （P〈0.2）. The least square analysis showed that the birds with BGA haplotype had significantly higher AFW and AFP than the birds with other haplotypes （P〈0.05）. The birds with AAG haplotype had significant higher LW and LW/BW than the birds with other haplotypes （P〈0.05）. The birds with AAG haplotype had significant higher PMiW and PMiW/BW than the birds with other haplotypes （P〈0.05）. The birds with AAG haplotype had significant higher ShL,FeW,MeL,MeC and KeL than the birds with AGG haplotypes （P〈0.05）. 【Conclusion】 The haplotype-based correlations reve

关 键 词：鸡 PPARΓ 单倍型 脂肪 骨骼 主效基因 

分 类 号：S831[农业科学—畜牧学]