基于透射和反射时间分辨测量数据的荧光参数三维重建  

Reconstructing Three-Dimensional Fluorescent Parameters Using Time-Resolved Data Based on Transmittance and Reflection Measurements

作　　者：张丽敏[1] 高峰[1] 李娇[1] 赵会娟[1] 

机构地区：[1]天津大学精密仪器与光电子工程学院,天津300072

出　　处：《中国激光》2009年第10期2552-2556,共5页Chinese Journal of Lasers

基　　金：国家863计划(2009AA02Z413);国家自然科学基金(60578008);天津市自然科学基金(07JCYBJC06600);高等学校学科创新引智计划(B07014)资助课题

摘　　要：提出了一种同时重建荧光参数的全三维时域荧光分子层析方法,通过选择一对合适的拉普拉斯变换因子提取时间扩展曲线的特征信息,求解时域耦合扩散方程。在图像重建过程中,应用代数重建技术求解线性逆问题,且引入归一化玻恩比方法。基于多通道时间相关单光子计数系统对模拟仿体进行了透射和反射式时间分辨数据测量,利用所提方法同时重建了荧光产率和寿命的三维图像。实验结果表明利用反射式测量数据目标体位置、大小、形状等方面具有一定的偏差,透射式达到了较好的重建效果,而两种模式相结合重建效果最优。We present a full three-dimensional algorithm for time-domain fluorescence molecular tomography. A pair of appropriate transform-factors is employed to extract the featured information on the temporal profiles for time-domain coupled diffusion equations. The linear inversions at two distinct transform-factors are solved with an algebraic reconstruction technique,and the normalized Born ratio is proposed. The simulate phantom is measured in reflection and transmittance modes by use of multi-channel time-correlation single photon counting system. We experimentally validate that the proposed scheme can achieve simultaneous three-dimensional reconstruction of the fluorescent yield and lifetime. The results show that for the positions,sizes and shapes of the targets,there are some deviation in reflection measurement,the quality in transmittance one is better and in the two-mode is more satisfied.

关 键 词：生物光学 荧光分子层析 图像重建技术 时间分辨 

分 类 号：Q63[生物学—生物物理学]