单细胞水平的光纤共焦后向散射显微光谱  

Fiber Confocal Back-Scattering Micro-Spectrum in Single Biology Cellular Scale

作　　者：王成[1] 方宝英[1] 蔡文杰[1] 徐安成[1] 陈芸[2] 李延[2] 魏勋斌[2] 

机构地区：[1]上海理工大学医学光学与视光学研究所,上海200093 [2]复旦大学生物医学研究院,上海200032

出　　处：《中国激光》2009年第10期2636-2641,共6页Chinese Journal of Lasers

基　　金：上海市重点学科发展基金(B109);国家985二期;上海市教育发展基金(2007CG62);上海高校选拔优秀青年教师科研专项基金(571211)资助课题

摘　　要：为了在实验上分析人正常胃上皮细胞与癌变细胞的显微后向散射光谱的区别，为临床医学检测提供实验依据，在光纤共聚焦显微成像技术基础上，结合细胞散射理论，建立了一套基于光纤共聚焦的细胞检测显微光谱分析装置，能够同时获取细胞显微图像和显微光谱医学信息。利用本装置测量了贴壁人正常和癌变胃上皮细胞，得到细胞水平的显微光谱特性。波长在500～800nm，癌变细胞的散射光强明显高于正常细胞所对应的散射光强；正常细胞所对应的光谱曲线中，800～1000nm范围内有规则的强度变化，癌细胞没有出现这种干涉；说明本装置适用于细胞的癌变检测，而且实验结果与现有理论预测结果一致。理解不同病理阶段的细胞光散射特性有助于为在体实时检测早期癌变组织提供有利的光学手段。In order to analyze experimentally the back-scattering micro-spectrum difference between human normal stomach epithetic cell and the cancerous cell to provide optical technique for clinical checking, a micro-spectral measurement setup for checking cell was established based on fiber confocal microscopically imaging technique combined with cell light scattering theory. It could provide micro-imaging and micro-medical spectral information simultaneously. Adherent monolayer human normal gastric epithelium line GES-1 and human carcinoma cell line NCI- N87 were measured with the setup respectively. The spectral results illuminated that micro-back-scattering intensity from NCI-N87 cells was stronger than that from GES-1 in 500 -800 nm range, and GES-1 ＇ s scattering spectrum showed a regular intensity change but NCI-N87 ＇s didn＇t. It indicates that fiber confocal back scattering （FCBS spectrometer） micro-spectral measurement system established can distinguish normal from dyplastic cell at cellular level. The experimental results agree well with theoretical expecting results from literature. Understanding the cell scattering properties of different pathological phases will aid in the development of noninvasive optical techniques for detection of precancerous conditions.

关 键 词：光谱 散射 共聚焦 癌 细胞 

分 类 号：O436.2[机械工程—光学工程]