PRRSV SX-1株的分离与NSP2和ORF5基因的克隆及变异分析  

Isolation of Porcine Reproductive and Respiratory Syndrome Rirus and Mutation Analysis of Its Nsp2 and ORF5 Gene

作　　者：张金强[1,2] 吴家强[1] 朱向福 于美芳[1] 白华[1] 孟斌[2] 任素芳[1] 王文志[1] 王金宝[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所,山东济南250100 [2]山东农业大学,山东泰安271000 [3]新泰市青云街道办事处兽医站,山东新泰271201

出　　处：《家畜生态学报》2009年第5期24-28,共5页Journal of Domestic Animal Ecology

基　　金：山东省自然科学基金重点项目(Z2007D06);山东省重大科技专项(2007ZHZX11103);山东省农科院青年基金项目(2005YQ039)

摘　　要：从山东莘县恒顺猪场发病猪群中分离到PRRSV病毒(SX-1株)。用RT-PCR法对该分离株的NSP2和ORF5基因进行了扩增和克隆,并对其进行核苷酸序列测定和分析。结果表明,PRRSV SX-1株的NSP2发生30个氨基酸的不连续缺失,缺失位置与JXA1毒株相同;ORF5基因与JXA1、CH-1a、VR2332核苷酸同源性分别为98.8%、94.9%、88.9%,氨基酸同源性分别为99.0%、93%、87.5%。基因系统发育树分析结果显示,SX-1与JXA1、HUN株的亲缘关系很近,与CH-1a亲缘关系较近,与VR2332、CC-1、RespPRRS/ReproMLV等毒株处于不同分支。A strain of porcine reproductive and respiratory syndrome virus（PRRSV）was isolated from Hengshun pig herd of Xin county with high fever syndrome in Shandong and it was designated as SX-1 strain. The NSP2 gene and ORF5 gene of SX-1 strain were amplified by RT-PCR and sequenced. The results showed that SX-1 strain was classified into the North American and it had discontinuous 30 amino acids deletions in NSP2. The ORF5 of SX-1 strain shared 98.8% ,94.9% and 88. 9% of nueleotide homology , and 99% ,93%,64.3% of amino acid identity , with those of JXA1, CH-1a and VR2332. The phylogenetic tree revealed SX-1 had closer relationship with JXA1 and HUN strains than that of CH-1a strain.

关 键 词：猪繁殖与呼吸综合征病毒 分离 NSP2基因 ORF5基因 变异分析 

分 类 号：S811.5[农业科学—畜牧学]