12种二肽衍生物的非水毛细管电泳分离研究  

Study on Separation of 12 Derivatized Dipeptides by Nonaqueous Capillary Electrophoresis

作　　者：王延宝[1,2] 王明清[2] 白新伟[2] 尤进茂[2,3] 

机构地区：[1]济宁学院化学系,山东曲阜273165 [2]曲阜师范大学化学与化工学院,山东曲阜273165 [3]中国科学院西北高原生物研究所,青海西宁810001

出　　处：《温州大学学报（自然科学版）》2009年第5期46-51,共6页Journal of Wenzhou University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20075016)

摘　　要：以新型荧光试剂2-(11H-苯[a]咔唑)乙基氯甲酸酯(BCEC-Cl)为柱前衍生试剂,采用非水毛细管电泳技术对衍生二肽进行分离.以甲醇和乙腈为溶剂,乙酸-乙酸铵为缓冲体系,采用279nm二极管阵列检测.在甲醇与乙腈体积比为7:3、乙酸铵浓度为50mmol·L-1、乙酸浓度为0.8mol·L-1、柱温为25℃、分离电压为28kV的条件下,实现了12种二肽衍生物的基线分离.Using a new fluorescent reagent 2-（11H-benzo[a]carbazol-11-yl）ethyl carbonochloridate （BCEC-Cl） as derivatization reagent, the separation of derivatized dipeptides could be achieved by nonaqueous capillary electrophoresis. In the experiment, an admixture of methyl alcohol and methyl cyanide was used as the background electrolyte solvent, an admixture of acetic acid and ammonium acetate were used as the buffer solution and diode array detection at 279 nm was used. Under the optimum experimental condition, namely, 7：3 （V/V） proportion of methyl alcohol and methyl cyanide, 50 mmol.L^-1 of ammonium acetate and 0.8 mol.L^-1 acetic acid, 25℃ column temperature, 28 kV voltage, a satisfactory baseline separation of 12 derivatized dipeptides could be obtained.

关 键 词：二肽 2-(11H-苯[a]咔唑)乙基氯甲酸酯(BCEC-Cl) 非水毛细管电泳分离 

分 类 号：O657.8[理学—分析化学]