正交实验法优化灵芝多糖快速提取工艺  

Technological Procedures of Rapid Extraction of Polysaccharide from Ganoderma Lucidum

作　　者：赵景辉[1] 王再幸[1] 赵伟刚[1] 李荣峰[1] 徐志臣[1] 赵明忠[1] 郭崇民[1] 潘扬[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2009年第3期28-30,共3页Special Wild Economic Animal and Plant Research

基　　金：科技部农业科技成果转化基金项目(2007GB23260409);科技部科研院所技术开发研究专项资金项目(NC-STE-2007-JKZX-292)

摘　　要：目的对灵芝多糖的快速提取工艺方法进行了优化,为工业化生产提供可靠的实验数据和理论依据。方法采用正交试验设计提取方法,氧化酶反应比色法测定多糖含量。结果最佳提取工艺为A3B2C3。影响因素的大小依次为:脉冲数、料液比、电场强度。此法提取的灵芝中多糖含量为0.416%,得膏率达6.23%。结论本提取工艺可行,经济、省时、回收率高。Objective To investigate a kind of technology of rapid extraction of polysaccharide from Ganoderma Lucidum in order to provide some reliable experimental data and theoretical basis for batch production.Methods It was determined for the polysaccharide content by orthogonal experimental design to the extraction technique and oxidize enzyme colormetric analysis.Results The best extract technology is A3B2C3;influence factors are electric field intensity, ratio to raw material, liquid and pulse frequency in order;the rate for extraction up to 0.416%, and rate of getting medicinal extract is 6.23%.Conclusions The technology above with high recycling rate is feasible and economic and saving time at the sametime.

关 键 词：灵芝 多糖 提取工艺 正交实验 

分 类 号：S567.31[农业科学—中草药栽培]