酶联免疫法分析糖尿病患者血清人巨细胞病毒抗体水平  

Enzyme-linked Immunosorbent Assay in Analysis of Human Cytomegalovirus Antibody in Diabetic Patients

作　　者：林伟良[1] 刘文虎[1] 徐冉行[2] 凌志强 

机构地区：[1]浙江省宁波市江东区明楼街道社区卫生服务中心,315040 [2]浙江省宁波市第六医院 [3]Shiga University of Medical Sciences,Japan

出　　处：《中国全科医学》2009年第20期1838-1840,共3页Chinese General Practice

基　　金：浙江省医药卫生科学研究基金项目(2005B156)

摘　　要：目的探讨人巨细胞病毒(HCMV)感染与糖尿病的关系。方法利用酶联免疫法(捕获酶联法和间接酶联法)检测727例糖尿病(85例1型糖尿病,642例2型糖尿病)患者及230例健康体检者(正常对照组)血清HCMV-IgM、IgG、IgA水平,并比较不同性别、年龄、病程的糖尿病患者HCMV-IgM、IgA、IgG的阳性率。结果1型糖尿病患者HCMV-IgM、IgA及IgG阳性率分别为10.59%、11.76%、92.94%及2型糖尿病患者HCMV-IgM、IgA及IgG阳性率分别为11.21%、11.68%、92.83%与正常对照组分别为0.87%、1.30%、77.39%比较,差异均有统计学意义(P<0.05)。年龄<30岁及病程<1年的糖尿病患者,血清HCMV-IgG阳性率与其他年龄组、病程组的患者比较,差异均有统计学意义(P<0.05)。结论HCMV感染在糖尿病进展中起着重要作用,年龄小或病程短的糖尿病患者既往感染者少。Objective To explore the relationship between HCMV infection and diabetes.Methods Levels of HCMV-IgM,IgG,IgA were determined by enzyme-linked immunosorbent assay in 727 diabetic patients（85 with type Ⅰ,642 with type Ⅱ） and 230 healthy subjects（normal control group）.Results The positive rates of HCNV-IgM,IgA,IgG were 10.59%,11.76%,92.94%,respectively,in type Ⅰ group,and 11.21%,11.68%,92.83%,respecitively,in type Ⅱ group,and 0.87%,1.30%,77.39%,respecitively,in control,the difference was significant（P〈0.05）.Patients less than 30 years old or with below-1-year course were significant different from other age groups or patients with other disease courses in HCMV-IgG positive rate（P〈0.05）.Conclusion Primary HCMV infection rates are significantly higher in younger patients and/or patients having recently suffered from diabetes mellitus.HCMV infection is important in the pathogenesis of diabetes mellitus and its mechanism should be further studied.

关 键 词：糖尿病 酶联免疫吸附测定 巨细胞病毒 

分 类 号：R587.1[医药卫生—内分泌]