吹扫-捕集-气质联用法分析测定侧柏挥发物  

Determination of volatiles in Platycladus orientalis L.phloem by a purge and trap injector-GC/MS

作　　者：武晓颖[1] 许志春[2] 关玲 金幼菊[1] 陈华君[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083 [3]北京市林业保护站,北京100029

出　　处：《生态学报》2009年第10期5708-5712,共5页Acta Ecologica Sinica

基　　金：北京市科委资助项目(D0705002040391);长江学者和创新团队发展计划资助项目(IRT0607)

摘　　要：采用吹扫捕集-气质联用法分析了侧柏(Platycladus orientalisL.)韧皮部的挥发物,并与常用的热脱附-气质联用法进行了比较。结果表明,仅用0.2g材料,吹扫5min即可得到较好的分离鉴定结果,通过增加2min反吹时间能明显降低空气峰和水峰的影响;该方法测得的各组分与热脱附法测的组分种类相同,均检测到9种单萜和3种倍半萜,但各组分在相对含量上有差异。与热脱附法相比,吹扫捕集法具有采样量少,不受吸附剂干扰和不受穿透体积影响的特点,有利于低沸点挥发物的测定,适于针叶植物挥发物的快速检测。A purge ＆ trap injector（PTI）-GC/MS was used to detect volatiles in Platycladus orientalis L.phloem.Data were compared with the thermo-desorption ＆ cold trap（TCT）-GC/MS method.Results showed that sample quantity of 0.2g and purge time of 5 minutes were adequate for separation and identification,and that backflash time of 2 minutes was sufficient for effectively removing the water vapor from the air and deicing the trap.Nine monoterpenes and three sesquiterpenes were identified by using both methods.Compared with TCT-GC/MS,PTI-GC/MS has the advantages of less quantity of samples required,no interference by absorbent,no thermal decomposition,and more sensitive to the monoterpenes,making it effective for rapid and accurate determination of conifer volatiles.

关 键 词：吹扫捕集-气质联用法 热脱附-气质联用法 侧柏韧皮部 挥发物 

分 类 号：S791[农业科学—林木遗传育种]