珍珠滴丸微生物限度检查方法学验证研究  

Study on Verification of Pearl Pill's Microbial Limit Test Methods

作　　者：陈万里 李勇军[2] 王永林[2] 

机构地区：[1]贵州省药品检验所,贵州贵阳550004 [2]贵阳医学院药学院,贵州贵阳5500042

出　　处：《抗感染药学》2009年第3期164-167,共4页Anti-infection Pharmacy

基　　金：贵州省中药现代化科技产业研究开发专项基金项目(No.黔科合农字〔2005〕5040)

摘　　要：目的:探讨珍珠滴丸的微生物限度检验方法,消除微生物限度检查法中珍珠滴丸本身的抑菌作用。方法:按平皿法和薄膜过滤法,在样品中加入试验菌,测定试验菌的回收率。结果:采用薄膜过滤法,各试验菌在样品中的回收率达70%以上,而平皿法试验菌的回收率未能达到试验要求。结论:采用薄膜过滤法能够更好的消除珍珠滴丸本身的抑菌作用,使测定结果准确,达到检测目的。Objective： To investigate the microbial limit test methods of pearl pill in order to eliminate the antimicrobial effect of pearl pill in the microbial limit test. Methods： By plate method and membrane filtration method in the sample adding test bacteria, determining the test bacteria recovery. Results： Using membrane filtration method, the test samples have reached the recovery rate of more than 70 percent. The recovery by the plate method can not be reached. Conclusion： Using membrane filtration method is better to eliminate the pearl pill inhibitory role to enable accurate determination and to reach the test purposes.

关 键 词：珍珠滴丸 微生物 限度检查 验证 

分 类 号：R915[医药卫生—微生物与生化药学]