SOD对γ射线辐照枯草芽孢杆菌的保护效应研究  

Protective effects of SOD on Bacillus subtilis irradiated by γ-rays

作　　者：柳芳[1] 陈晓明[1] 李晓燕 朱捷[1] 郑春 严万里[1] 周莉薇[1] 李承龙[1] 胡玉雪[1] 周斌[1] 陆振渊[1] 朱海蓝[1] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]中国物理工程研究院核物理与化学研究所,绵阳621900

出　　处：《核技术》2009年第10期774-778,共5页Nuclear Techniques

基　　金：中国工程物理研究院科学技术发展基金(2008A0103002);西南科技大学核废物与环境安全国防重点学科实验室基金项目(07JGZB07)资助

摘　　要：为了考查外源SOD对γ射线辐照微生物的保护效应,选用不同剂量γ射线辐照外源SOD处理过的枯草芽孢杆菌营养体,采用平板计数法考查辐照后的细胞存活率,黄嘌呤氧化法检测胞内SOD活性,脉冲场凝胶电泳分析DNA双链断裂水平的变化。结果表明,不同浓度外源SOD均可使γ射线辐照后的细胞存活率明显增加;营养体胞内残留SOD活性与外源SOD浓度以及辐照剂量之间的关系没有明显的变化规律;外加SOD使细胞DNA双链断裂水平(PR)显著下降。并且细胞存活率和PR值随外源SOD浓度以及辐照剂量变化的趋势基本一致。研究结果显示外源SOD在γ射线辐照中对枯草芽孢杆菌有保护效应,且SOD的保护效应与其浓度呈一定相关。In order to investigate the protective effect of SOD, Bacillus subtilis vegetative cells treated with SOD of different concentrations were irradiated to different doses by ^60Co γ-rays. Cell survival rate was examined with the standard plate-count method. Intercellular SOD activity was measured by SOD kit with xanthine oxidase method, and DNA double-strand breaks was analyzed by pulsed-field gel electrophoresis （PFGE）. The results showed that the cell survival rate increased obviously with the SOD-added Bacillus subtilis, though we did not find a clear relation among the intercellular SOD activity, concentration of added SOD and irradiation dose. The DNA release percentage （PR） value decreased evidently with the SOD-treated Bacillus subtilis. Basically, the trend of cell survival rate with concentration of the added SOD and irradiation dose is consistent with the PR value. It can be concluded that exogenous SOD could protect Bacillus subtilis vegetative cell from damage induced by y-rays, and the protective efficiency of added SOD is related to the concentration.

关 键 词：超氧化物歧化酶 枯草芽孢杆菌 辐照抗性 DNA双链断裂 

分 类 号：Q142.6[生物学—生态学] Q691.8[生物学—普通生物学]