聚乙二醇辛基苯基醚胶束增敏荧光分光光度法测定人尿中痕量1-羟基芘  

Fluorospectrophotometric Determination of 1-Hydroxypyrene in Human Urine with Micellar Sensitization of Triton X-100

作　　者：欧阳运富[1] 王永生[2] 唐宏兵[1] 

机构地区：[1]常州市疾病预防控制中心理化检验科,常州213003 [2]南华大学公共卫生学院,衡阳421001

出　　处：《理化检验（化学分册）》2009年第10期1194-1196,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：湖南省自然科学基金资助课题(03JJY3030)

摘　　要：在pH 8.0的B-R缓冲溶液中,加入适量表面活性剂Triton X-100,可引起1-羟基芘的荧光显著增强,在3.2～982.0μg·L^(-1)范围内荧光强度与1-羟基芘的质量浓度呈线性关系。方法的检出限(3S/N)为1.0μg·L^(-1)。据此,提出了测定人尿中1-羟基芘的新方法。方法的加标回收率为93.2%～100.7%范围,对200.0,600.0μg·L^(-1)1-羟基芘标准溶液平行测定7次,相对标准偏差分别为1.78%和0.98%。应用于人尿中痕量1-羟基芘的测定,结果与高效液相色谱法一致。It was found that in a B-R buffer medium of pH8.0, the fluorescence intensity of 1-hydroxypyrene （1-OHP in short） was significantly sensitized by the Triton X-100 micelle, and linear relationship was obtained between values of fluorescence intensity and mass concentration of 1-OHP in the range from 3. 2 to 982. 0 μg · L^-1 when measured at the wavelengths of （λex） 279 nm and （λem） 390 nm Detection limit （3S/N） of the method found was 1.0μg ·L^-1. Based on these facts, a method for determination of 1-OHP in human urine was proposed Precision of the method was tested by replicate determination, and values of RSD （n=7） found were 1. 78% for 200μg·L^-1 of 1-OHP and 0. 98% for 600μg·L^-1 of 1-OHP. Recovery was tested by standard addition method on the base of human urine samples as matrixes, and values of recovery obtained were ranged from 93. 2% to 100. 7%. Results obtained by the present method were checked very well with the results found by HPLC.

关 键 词：荧光分光光度法 1-羟基芘 胶柬增敏 TRITON X-100 人尿 

分 类 号：O657.31[理学—分析化学]