番荔枝DNA的提取和AFLP体系的建立  

DNA Extraction from Sweetsop (Annona squamosa L.) and Establishment of AFLP Analysis System

作　　者：赵志常[1] 胡桂兵[2] 刘运春[2] 欧阳若[2] 罗石荣[3] 

机构地区：[1]四川大学生命科学学院,四川成都610065 [2]华南农业大学园艺学院,广东广州510642 [3]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《北方园艺》2009年第10期44-47,共4页Northern Horticulture

基　　金：海南省自然科学基金资助项目(80504)

摘　　要：以7份番荔枝品种为试材,通过对DNA的提取、酶切、连接、预扩增和选择性扩增等分析,建立并研究番荔枝的AFLP分析体系,以其找出番荔枝品种间的亲缘关系,为番荔枝的杂交育种的亲本选择和嫁接砧中砧穗选择提供理论基础。结果表明:利用该体系对7份番荔枝的样品进行了分析,可以获得清晰的条带,表明所建立的体系可使用于番荔枝AFLP分析。Studying family relationship in sweetsop germplasm resources, in order to select parents in sweetsop breeding and grafted rootstock. The reaction system of AFLP in 7 sweetsop germplasm resources was studied, and analysis system was established by detected the results of several reactions, such as DNA extraction,enzyme restriction,adaperligation preamplification and selective amplification. The several germplasm resources of sweetsop were studied by using AFLP technique with primer EcoRI-CAG＋MseI-CGA, and clear amplification bands were obtained. The results showed that the reaction system could be used for AFLP analysis of sweetsop.

关 键 词：番荔枝 DNA提取 AFLP 

分 类 号：S667.9[农业科学—果树学]