金属硫蛋白基因转化黄瓜的研究  

Study on Genetic Transformation of Cucumber(Cucumis sativus L.) with Metallothionein Gene

作　　者：郑伟[1] 杨苹[1] 李洪志[1] 王艳芳[1] 徐岩[1] 庞实锋[1,2] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118 [2]中山大学生命科学学院,广东广州510275

出　　处：《北方园艺》2009年第10期58-61,共4页Northern Horticulture

基　　金：十一五"863"计划生物反应器重大专项资助项目(2007AA100503);吉林省科技发展重点计划资助项目(20070922);高等学校科技创新工程重大项目培育资金资助项目(70S018);长春市科技计划资助项目(06GG150)

摘　　要：以黄瓜无菌苗子叶切段为外植体,通过叶盘转化法与根瘤农杆菌进行共培养建立了黄瓜的遗传转化体系。比较了不同苗龄,不同侵染时间对黄瓜离体再生的影响,并做了潮霉素敏感性测试。结果表明：苗龄3-4 d的子叶,侵染时间为15 min,潮霉素浓度为25 mg/L最适分化出抗性小苗。最终获得3株抗性植株,经过PCR和RT-PCR检测,初步确定均为阳性转化植株。In this experiment, we chose cotyledon segments of Cucumis sativus L. as explants, and transformation was mediated by Agrobacterium tumefaciens. We compared the effect of different seeding age, different infection time on cucumber in vitro regeneration, and made a test of hygromycin for screening. The results were as follows： seeding age were 3 or 4 day, infection time was 15 minutes, the concentration of hygromycin was 25 mg/L, could best obtain putative transgenic adventitious bud differentiation. Finally, we got three putative transgenic plants, they were all showed the positive transformed plants detected by PCR and RT-PCR.

关 键 词：黄瓜 金属硫蛋白(MT) 基因 愈伤 绿色荧光蛋白(GFP) 转化 

分 类 号：S642.203.6[农业科学—蔬菜学]