利用多因子正交试验建立桑的无菌快繁体系  

Application of Orthogonal Design in Establishing Sterile Rapid Propagation System of Mulberry

作　　者：李想韵[1] 朱鸿[1] 饶灿[1] 李鹏[1] 宋锋[1] 

机构地区：[1]西南大学生命科学学院,四川重庆400715

出　　处：《北方园艺》2009年第10期90-92,共3页Northern Horticulture

基　　金：三峡库区生态环境教育部重点实验室开放基金资助项目(EF200609)

摘　　要：以桑侧枝茎段为材料,利用L9(34)正交设计探讨培养基种类和激素配比对桑茎段丛生芽诱导效率的影响,并对影响桑外植体生根的主要因子进行了研究。结果表明:A2B2C1为诱导出芽的最佳培养基与激素组合,并且可同时实现芽增殖;生根培养基为WPM+1.0 mg/L IBA。The lateral branches of Mulberry were taken as explants,effects of various medium and different plant hormones on caespitose buds induction was studied by L9（34） orthogonal design,and the roots induction was researched.The results showed that： the optimal medium for buds induction was A2B2C1,meanwhile the breed up of casespitose buds was realized;the cultural medium for growth of roots was WPM＋1.0 mg/L IBA.

关 键 词：桑 正交设计 不定芽诱导 生根 

分 类 号：S686[农业科学—观赏园艺]