斑点免疫结合试验斑点免疫金渗滤试验和培养法用于解脲支原体检测的对比研究  

Comparison of dot immunobind assay,dot immunogold filtration assay and culture for the detection of ureaplasma urealyticum.

作　　者：何云志[1] 郭秀军[1] 张国威[1] 

机构地区：[1]第三军医大学新桥医院皮肤科

出　　处：《中国皮肤性病学杂志》1998年第6期363-365,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的：探讨斑点免疫结合试验（ＤＩＢＡ）、斑点免疫金渗滤试验（ＤＩＧＦＡ）和培养法检测解脲支原体的优缺点。方法：同时用ＤＩＢＡ、ＤＩＧＦＡ和培养法检测疑诊非淋菌性尿道炎、阴道炎或宫颈炎患者泌尿生殖道标本中的解脲支原体，并相互比较。结果：ＤＩＢＡ和ＤＩＧＦＡ两法的敏感性、特异性分别为９２．３％、９３．８％和９４．２％、９６．４％。配对计数资料χ２检验，两法分别与培养法比较均无显著性差别（Ｐ＞０．０５）。结论：ＤＩＧＦＡ具有较为省时和操作简便的优点，是一种有效、快速和简便的检测方法，具有广泛应用于临床实验室检测解脲支原体感染的潜力。Objective:To assess dot immunobind assay(DIBA),dot immunogold filtration assay(DIGFA) and culture for the detection of ureaplasma urealyticum. Methods:167 urogenital specimens were obtained from patients with suspected nongonococcal urethritis or cervicitis and vaginilis.These specimens were tested by DIBA,DIGFA and compared with culture for detection of ureaplasma urelyticum.Results:The sensitivities and specificities of the two assays were 92.3%,93.8% respectively,and 94.2%,96.4% respectively.The results of paired comparison χ 2 test indicated that the difference between both of the two assays and culture were not significant ( P >0.05).Conclusion:DIGFA had the advantage of saving time and easier operation.DIGFA would be a useful,esay and rapid method and have potential application used widely in the clinical laboratory to detect ureaplasma urealyticum infection.

关 键 词：解脲支原体 DIBA DIGFA 

分 类 号：R446.61[医药卫生—诊断学]