重症急性胰腺炎细菌移位的重组质粒标记研究  

The Study on Bacterial Translocation in Severe Acute Pancreatitis with Labeling of Recobinant Plasmid

作　　者：陈建中[1,2] 戴植本[1,2] 

机构地区：[1]同济医科大学附属同济医院外科 [2]汕头大学医学院第一附属医院外科

出　　处：《中国普通外科杂志》1998年第6期333-336,共4页China Journal of General Surgery

摘　　要：利用重组质粒转化大肠杆菌作标记，对重症急性胰腺炎时大鼠肠道细菌移位及肠形态学进行了研究。结果表明：重症急性胰腺炎发病后２～４小时可在腹腔内检及有活力的标记菌，１２～３６小时渐增至高峰，并可在光镜和电镜下发现微绒毛脱落，基底膜水肿等粘膜损害，甚至可在肠细胞胞浆内发现细菌，说明本病很早即可发生肠道细菌移位至腹腔各脏器。With escherichia coli which bears the recombinant plasmid, we studied bacteria translocation and intestinal morphology in rats of severe acute pancreatitis. The results showed that: living marked bacteria can be found in abdominal cavity as early as 2h to 4h after duction, it reaches the peak gradually from 12h to 36h. Denudated microvillus and edema of lamina propria were also observed under light microscope and electric microscope. We even observed bacteria which were within cytoplasm of intestinal cell. All these findings supported that bacteria which exist in intestinal tract can migrate to every organ in abdominal cavity, it revealed that bacterial translocation may lead to secondary pancreatitis.

关 键 词：急腹症 胰腺炎 细菌移位 质粒 病理 

分 类 号：R657.510.2[医药卫生—外科学]