淫羊藿苷对大鼠骨髓间充质干细胞向成骨细胞分化的影响  

Effects of icariin on osteogenic differentiation of rat bone marrow mesenchymal stem cells

作　　者：蒋绍艳[1] 宋丹妮[1] 史玉朋[2] 常宏[1] 

机构地区：[1]深圳市妇幼保健院药剂科,广东深圳518028 [2]深圳市龙岗中心医院骨科,广东深圳518116

出　　处：《海南医学院学报》2009年第10期1198-1200,共3页Journal of Hainan Medical University

基　　金：深圳市科技局课题(200802068)~~

摘　　要：目的:探讨淫羊藿苷对大鼠骨髓间充质干细胞成骨分化的影响。方法:采用差速贴壁法体外分离培养大鼠骨髓间充质干细胞,流式细胞仪鉴定表面标志。取第3代细胞加入10-6mol/L淫羊藿苷培养基,对照组仅加入等量普通培养基进行培养。通过镜下观察细胞形态,流式细胞仪测定细胞表面标志,碱性磷酸酶试剂盒测定诱导细胞碱性磷酸酶活性,组织化学染色观察钙化结节形成能力。结果:体外培养的细胞表达CD29、CD44、CD105、CD166。淫羊藿苷诱导组细胞碱性磷酸酶呈阳性反应并有钙化结节形成,而对照组未见钙化结节形成。结论:淫羊藿苷能够诱导大鼠骨髓间充质干细胞向成骨细胞分化,可视为一种良好的骨诱导活性因子。Objective： To study the effects of icariin on osteogenic differentiation of rat BMSCs.Methods： Rat BMSCs were isolated and cultured in vitro.At the third passage,rat BMSCs were treated with 10-6mol/L icariin medium.BMSCs in the control group were treated with an equal volume of medium.Cells were identified by flow cytometry.Alkaline phosphatase were detected by alkaline phosphatase kit,and Calcified nodules were observed using immunohistochemistry staining.Results： Flow cytometry analysis showed that BMSCs cultured in vitro expressed mesenchymal cell marker, including the positive expression of CD29 ,CD44 ,CD105 and CD166. The staining of the alkaline phosphatase and calcified nodules were positive after osteoinduction, but calcified nodules were not found in rat BMSCs in the control group. Conclusion： Icariin can induce osteogenic differentiation of rat BMSCs and can be used as an osteoinductive factor.

关 键 词：淫羊藿苷 骨髓间充质干细胞 成骨分化 

分 类 号：Q813.11[生物学—生物工程] R318[医药卫生—生物医学工程]