香蕉柠檬酸合酶基因MaGCS的克隆及生物信息学分析  

Cloning and Bioinformatic Analysis of MaGCS Encoding a Homolog Citrate Synthase from Banana

作　　者：迟光红[1,2] 周雪丽[1,2] 李美英[1,2] 徐碧玉[1] 金志强[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]海南大学农学院,海南儋州571737

出　　处：《热带农业科学》2009年第8期12-18,共7页Chinese Journal of Tropical Agriculture

基　　金：香蕉现代产业技术体系建设项目(No.nycytx-33)

摘　　要：采用RACE-PCR的方法首次在香蕉中获得一个柠檬酸合酶基因的cDNA序列。经序列测定和Blastx比对分析表明,该cDNA全长1885bp,编码513个氨基酸残基,具有植物柠檬酸合酶基因的特征结构域,并与其他植物来源的乙醛酸循环体的柠檬酸合酶具有很高的序列相似性,将其命名为MaGCS(Musa glyoxysomal citrate synthase),并对其进行生物信息学分析,初步预测其理化性质及功能等。A new cDNA homologous with citrate synthase genes was obtained by RACE-PCR from banana （Musa acurninoa L. AAA group ‘Brazil＇）. This cDNA was 1885bp in full length and encoded 513 amino acids. Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with glyoxysomal citrate synthase genes from other plants, and was designated as MaGCS （Mu.sa glyoxysomal citrate synthase）. Its bioinformation was analyzed to initially understand its physical and chemical properties and functions.

关 键 词：香蕉(Musa acuminate L.AAA group cv.Brazilian) 柠檬酸合酶基因 生物信息学分析 

分 类 号：S668.4[农业科学—果树学] Q785[农业科学—园艺学]