施氏假单胞菌UP-1降解二苯并噻吩的动力学模型  

KINETIC MODEL OF DIBENZOTHIOPHENE BIODEGRADATION BY Pseudomonas stutzeri UP-1

作　　者：侯影飞[1,2] 孔瑛[2] 郭宁[2] 李春虎[1] 

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100 [2]中国石油大学重质油国家重点实验室,山东青岛266555

出　　处：《石油学报（石油加工）》2009年第5期702-706,共5页Acta Petrolei Sinica(Petroleum Processing Section)

基　　金：国家自然科学基金项目(20676128)资助

摘　　要：对施氏假单胞菌(UP-1)降解二苯并噻吩(DBT)的过程进行了宏观动力学研究,以Michaelis-Menten方程为基础确定了UP-1降解DBT的动力学模型。计算得到动力学参数vmax=84.75 mg/(L.h),Km=759.59 mg/L。通过相关指数R2和F值检验,该动力学模型高度显著,2个重要参数取值可信。在考察的底物浓度范围内菌株UP-1降解DBT过程不存在底物抑制作用,但存在产物抑制作用。求得的UP-1休止细胞降解DBT的最大降解速率为88.5 mmol/(kg DC.h),其降解脱硫能力优异。The macro-kinetic study of dibenzothiophene （DBT） biodegradation by Pseudomonas stutzeri UP-1 was carried out. Based on Michaelis-Menten equation, the kinetics model of DBT degradation by UP-1 was obtained. The kinetic parameters of DBT degradation by UP-1, Vmax and Kin, were 84.75 mg/（L.h） and 759.59 mg/L, respectively. Being verified by correlation index R^2 and F value, the kinetics model had higher significance level and the value of the two important parameters was creditable. It was determined that there was no substrate inhibition action, but degradation products had anti competitive inhibition action for UP-1 to the degradation of DBT. It should also be noted that the maximum degradation rate of DBT by UP-1 was 88. 5 mmol/（kg DC. h）, meaning that UP-1 was a bacteria with high ability of desulfurization.

关 键 词：生物脱硫 二苯并噻吩 动力学模型 施氏假单胞菌UP-1 

分 类 号：Q819[生物学—生物工程]