绵枣儿核核糖体DNA ITS序列扩增的研究  

Study on Nuclear Ribosomal DNA ITS Amplification of Scilla scilloides(Lindl.)Druce

作　　者：尚彩玲[1] 丁开宇[2] 

机构地区：[1]山西中医学院,山西太原030024 [2]云南大学生命科学院,云南昆明650091

出　　处：《山西中医学院学报》2009年第4期52-54,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家自然基金资助项目(30270095)

摘　　要：采用通用引物扩增绵枣儿核核糖体DNA ITS序列,未得到目的条带。根据引物设计原则自行设计12个引物,其中只有使用引物对PA3、D(ITS3),PA4、D(ITS3),PA4、DO2扩增得到绵枣儿核核糖体DNA ITS序列。Universal primers were used to amplify nuclear ribosomal DNA ITS of SciUa scilloides （Lindl.） Druce,but they did not work.Then 12 primers were designed according to the principle of primer-design and were used .Nuclear ribosomal DNA ITS of Scilla seilloides （Lindl.） Druce was obtained using primers PA3 and D （ITS3）, PA4 and D （ITS3）, PA4 and DO2.

关 键 词：核核糖体 ITS序列 引物设计 

分 类 号：R318[医药卫生—生物医学工程]