白英水提物诱导人胃癌SGC-7901细胞凋亡的实验研究  

Study on Apoptosis of Human Stomach SGC-7901 Cells Induced by Solanum lyratum Thunb.Extract

作　　者：吴剑[1,2] 李华[1] 涂硕[1] 余乐涵[1] 万福生[1] 

机构地区：[1]南昌大学医学院,江西南昌330006 [2]江西医学院上饶分院,江西上饶334000

出　　处：《时珍国医国药》2009年第10期2509-2511,共3页Lishizhen Medicine and Materia Medica Research

基　　金：江西省科技攻关重点项目(No.060004);江西省卫生厅资助项目(No.2006A15)

摘　　要：目的观察白英水提物(SLT)对胃癌SGC-7901细胞凋亡及相关基因表达的影响。方法常规法制备白英水提物;实验分正常对照组、白英处理组和顺铂处理组。白英处理组加入白英水提物终浓度分别为12.5,25和50 mg/ml。MTT法检测细胞增殖抑制率,荧光显微镜观察SGC-7901细胞凋亡及形态变化,半定量RT-PCR分析基因bcl-xl,bid,caspase-9和caspase-3 mRNA表达。结果与正常对照组比较,3个剂量白英处理组SGC-7901细胞增殖抑制均有显著性增加,且呈明显的时效和量效关系,细胞凋亡呈显著性增多(P<0.05),表现细胞核固缩、染色质凝集、边聚等;基因bcl-xl mRNA表达显著性下降(P<0.05),bid、caspase-9和caspase-3 mRNA表达有显著性升高(P<0.05),并随白英浓度增加而加强。结论白英能剂量依赖性的抑制SGC-7901细胞增殖和诱导凋亡,其作用可能与调控bcl-xl、bid、caspase-9和caspase-3 mRNA表达相关。Objective To investigate the effects of Solanum lyratum Thunb.Extract（SLT） on apoptosis of human stomach cancer SGC-7901 cells.Methods Dried whole herbs of Solanum Lyratum Thunb were extracted by boiling distilled water.Human stomach cancer SGC-7901 cells were randomly divided into control group,SLT-treated groups（12.5 mg/ml,25 mg/ml,50 mg/ml） and positive control（25μg/ ml DDP）.The growth inhibitory rate was evaluated by MTT assay.Morphological changes of apoptosis were observed with fluorescence microscope.Cell apoptosis rate was determined by flow cytometry and the express of bcl-xl,caspase-9,caspase-3 and bid mRNA were detected by semi-quantity RT-PCR.Results Compared with control group,the cell proliferation inhibitory rate and apoptosis rate of Human stomach cancer SGC-7901 cells were increased obviously（P〈0.05） in SLT-treated groups.Morphology of SGC-7901 cells showed the nuclear shrinkage,chromatin condensation and margination,and no changes were observed in control group.The expression of bcl-xl mRNA was obviously decreased（P〈0.05）,the expression of caspase-9,caspase-3 and bid mRNA were obviously increased respectively（P〈0.05）,in a dose-dependent manner in SGC-7901 cells of the SLT-treated groups.Conclusion SLT can induce apoptosis and inhibit proliferation of the human stomach cancer SGC-7901 cells,by regulating expression of bcl-xl,caspase-9,caspase-3 and bid genes.

关 键 词：白英 SGC-7901细胞 凋亡 基因表达 

分 类 号：R285.5[医药卫生—中药学]