荧光定量PCR检测HBV-DNA与ELISA法乙肝免疫学检测的探讨  

Fluorescence Quantitative PCR Detection of HBVDNA and Immunological Detection of Hepatitis B by ELISA:A Correlation Study

作　　者：黄喜顺[1] 邱耀辉[1] 苏洪[1] 吴义森[1] 蓝宇频[1] 罗美瑄[1] 

机构地区：[1]解放军第一七五医院(厦门大学附属东南医院),福建漳州363000

出　　处：《中国现代医生》2009年第30期28-29,共2页China Modern Doctor

摘　　要：目的探讨HBV感染者乙肝免疫学检测与HBV-DNA的关系,为合理治疗乙肝患者提供较准确的依据。方法用ELISA方法检测HBV感染者乙肝免疫学检测,用PCR方法检测HBV-DNA,并对结果进行分析。结果HBsAg(+)HBeAg(+)HBcAb(+)组(大三阳组)患者血清HBV-DNA检出率97.06%(66/68),平均拷贝数为(2.15E+07)mL;HBsAg(+)HBeAb(+)HBcAb(+)组(小三阳组)患者血清HBV-DNA检出率为62.64%(57/91),平均拷贝数为(2.47E+04)mL;HBsAg(+)HBcAb(+)组血清HBV-DNA检出率为52.94%(18/34),平均拷贝数为(6.39E+04)mL。结论HBsAg和HBeAg阳性与HBV-DNA复制密切相关。乙肝免疫学检测和HBV-DNA检测将成为指导乙型肝炎防治的重要手段。Objective To explore the relationship between the immunological detection of hepatitis B in the HBV infected patients and the HBV-DNA in order to supply the accurate basis for the reasonable treatment of hepatitis B patients. Methods We used the immunological detection of hepatitis B of HBV infected patients by ELISA and the PCR detection of HBV-DNA,and analyzed the results. Results In HBsAg（＋） HBeAg（＋） HBcAb（＋） group,the patient blood serum HBV-DNA detection rate was 97.06%（66/68） and the average copy number was （2.15E＋07）mL. In HBsAg（＋） HBeAb（＋） HBeAb（＋） group, the patient blood serum HBV-DNA detection rate was 62.64%（57/91） and the average copy number was（2.47E＋04）mL. In HBsAg（＋） HBcAb（＋） group, the blood serum HBV- DNA detection rate was 52.94%（18/34） and the average copy number was（6.39E＋04）mL. Conclusion HbsAg and the HBeAg masculine gender is closely related to HBV-DNA duplication. Both the immunological detection of hepatitis B and the HBV-DNA detection will become an important means to guide prevention and treatment of hepatitis B.

关 键 词：ELISA法检测 荧光定量PCR检测 乙肝免疫学检测 HBV—DNA 

分 类 号：R446.1[医药卫生—诊断学] R512.6[医药卫生—临床医学]