小鼠静脉内注射白细胞介素8对动员骨髓造血干/祖细胞的作用研究  

Effect of intravenous injection of interleukin 8 on mouse hematopoietic progenitor cell mobilization

作　　者：张毅[1,2,3,4] 朱剑昆[1,2,3,4] 张雁云[1,2,3,4] 刘继明 邱玉华[1,2,3,4] 李新燕 刘蓓岭[1,2,3,4] 谢炜 郑列琳[1,2,3,4] 冯丽瑾 郭玲[1,2,3,4] 朱学东 强亦忠[1,2,3,4] 张学光 

机构地区：[1]苏州医学院免疫研究室 [2]苏州医学院生物化学教研室 [3]苏州市第二人民医院检验科 [4]苏州医学院放射医学研究所

出　　处：《中华血液学杂志》1998年第12期642-645,共4页Chinese Journal of Hematology

基　　金：江苏省科委优秀青年教师基金;国防科工委基金

摘　　要：目的：研究重组人白细胞介素８（ｒｈＩＬ８）动员小鼠造血干／祖细胞的生物学效应。方法：将ｒｈＩＬ８单独或联合重组人粒细胞集落刺激因子（ｒｈＧＣＳＦ）给ＢＡＬＢ／ｃ小鼠静脉内注射，观察ＢＡＬＢ／ｃ小鼠外周血单个核细胞（ＰＢＭＮＣ）的增殖能力；并将ＰＢＭＮＣ回输给经致死性照射（７．５Ｇｙ６０Ｃｏγ射线照射）的ＢＡＬＢ／ｃ小鼠，观察其造血重建功能。结果：每只小鼠单独注射ｒｈＩＬ８３０μｇ１５分钟后，外周血中混合细胞集落形成单位（ＣＦＵＭｉｘ）数量达峰值［（１９４±６１）×１０３／Ｌ］，比对照组［（７±３）×１０３／Ｌ］明显升高；外周血中ｃｋｉｔ＋Ｓｃａ１＋细胞为（３１８６±５１７）×１０３／Ｌ，亦比对照组［（６４４±３４１）×１０３／Ｌ］明显升高；联合注射ｒｈＧＣＳＦ则可使外周血ＣＦＵＭｉｘ和ｃｋｉｔ＋Ｓｃａ１＋细胞分别增高至（７９０±４８）×１０３／Ｌ和（１０７２９±２３３９）×１０３／Ｌ。单独注射ｒｈＩＬ８后动员的ＰＢＭＮＣ回输给致死性照射的ＢＡＬＢ／ｃ小鼠，其外周血中骨髓造血重建活性（ＭＲＡ）细胞比对照组明显升高，联合ｒｈＧＣＳＦ组外周血中ＭＲＡ细胞数可达（３５６．３±８７．７）×１?Objective: To study the mobilization effects of recombinant human IL 8(rhIL 8) on mouse peripheral blood hematopoietic progenitors (HPCs). Methods:A daily single dose of rhIL 8 was intravenously injected into normal or G CSF treated mice for two days. The number of colony forming unit Mix (CFU Mix) in peripheral blood was analyzed by colony assay, while the number of c kit +Sca 1 + HPCs was examined by immunofluorescence staining. In addition, colony forming unit of spleen (CFU S) and marrow repopulating ability (MRA) cells were examined by transplanting lethally irradiated mice. Results: Single dose of rhIL 8 (30μg) significantly increased the numbers of CFU Mix[(194±61)×10 3/L] and c kit + Sca 1 + cells [(3186±517)×10 3/L] in the peripheral blood as compared with that of normal saline controls [(7±3)×10 3/L and (644±341)×10 3/L] at 15 minutes after intravenous injection. G CSF could also increase peripheral CFU Mix and c kit + Sca 1 + cells to (790±48)×10 3/L and (10729±2339)×10 3/L, respectively, after subcutaneously injection for two days, this increase was further augmented by rhIL 8 injection. IL 8 alone or in combination with G CSF could significantly increase the number of CFU S and MRA cells in circulating blood. Conclusion: IL 8 alone or in combination with G CSF could significantly mobilize circulating HPCs.

关 键 词：白细胞介素8 G-CSF 造血干细胞 造血干细胞移植 

分 类 号：R457[医药卫生—治疗学] R973[医药卫生—临床医学]