茜草糖蛋白QC的分离纯化及结构探讨  

Isolation, purification and structure investigation of glycoprotein QC from Rubia cordifolia L.

作　　者：王红霞[1] 马百平[1] 屠爱萍[1] 张慧娟[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《军事医学科学院院刊》1998年第4期277-280,共4页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的：提取分离茜草（ＲｕｂｉａｃｏｒｄｉｆｏｌｉａＬ．）中的活性糖蛋白，并进行结构分析。方法：用ＤＥＡＥ－纤维素和ＳｅｐｈａｄｅｘＧ－１５０柱层析纯化茜草粗多糖，得到糖蛋白ＱＣ。用高效液相色谱法（ＨＰＬＣ）（ＩｏｎｐａｋＳ－８０５柱）检查纯度并测定相对分子质量。用薄层层析色谱法（ＴＬＣ）、气相色谱法（ＧＣ）、气－质联用色谱法（ＧＣ－ＭＳ）及红外光谱（ＩＲ）分析其多糖的结构。结果：糖蛋白ＱＣ经ＨＰＬＣ检查为单一对称峰，说明其为均一糖蛋白，ＱＣ的平均相对分子质量为４０１６８９；其蛋白含量为７．６％。ＴＬＣ和ＧＣ分析表明，ＱＣ由Ｒｈａ、Ａｒａ、Ｘｙｌ、Ｍａｎ、Ｇｌｃ、Ｇａｌ及半乳糖醛酸组成，其中性单糖摩尔比为１．００∶１．２９∶１．８６∶０．９９∶２．９７∶３．０４。ＩＲ分析表明，其含β型糖甙键。ＧＣ－ＭＳ分析表明，Ｇａｌ、Ｇｌｃ各６种连接方式，Ｍａｎ、Ｘｙｌ、Ａｒａ、Ｒｈａ分别有３、１、２、２种连接方式。各糖基的连接顺序有待于进一步的研究。结论：茜草中分离得到的糖蛋白ＱＣ是一种由多种糖残基组成的多分支的杂多糖蛋白，初步药理实验表明其具有明显的清除自由基的作用，清除率为９３．２４％。Objective: To isolate the bioactive glycoprotein from Rubia cordifolia L. and to elucidate its structure. Methods: Glycoprotein QC was isolated and purified by decocting with water,alcohol precipitation, ion exchange (DEAE cellulose) and gel filtration (Sephadex G 150) chromatography. Its homogeneity was proved by HPLC on Ionpak S 805 column. Its structure was analyzed by TLC, GC, GC MS and IR spectrometry. Results: The average MW of QC was 401 698 . According to TLC and GC analysis, the monosaccharide residues of QC were rhamnose, arabinose, xylose, mannose, glucose, galactose and galacturonic acid. The molar ratio of neutral sugars was 1.00∶1.29∶1.86∶0.99∶2.97∶3.04. GC MS Analysis showed that QC was highly branched in structure. IR Indicated that it contains β glucoside bond. Conclusion: QC Is a complex branched glycoprotein composed of several kinds of monosaccharide residues from Rubia cordifolia L. Preliminary pharmacological tests showed that QC had strong scavenging effects on free radicals with a scavenging rate of 93.24%.

关 键 词：茜草 糖蛋白 分离 纯化结构 

分 类 号：Q949.781.1[生物学—植物学] Q946.103