靶向p21的shRNA对姜黄素诱导肝癌细胞Huh7凋亡的影响  

Effect of p21-targeted shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells

作　　者：王伟章[1] 张碧鱼[2] 梅文杰[1] 毛建文[1] 李明[1] 

机构地区：[1]广东药学院,广东广州510006 [2]中山大学珠海校区基础教学中心,广东珠海519082

出　　处：《药学学报》2009年第10期1102-1106,共5页Acta Pharmaceutica Sinica

基　　金：广东省医学科研基金资助项目(A2008316);广东药学院重点培养青年教师基金资助项目

摘　　要：探讨靶向p21的shRNA对姜黄素诱导肝癌细胞Huh7凋亡的影响。采用RT-PCR和Western blotting方法检测姜黄素对肝癌细胞Huh7中p21 mRNA和蛋白表达的影响;构建针对p21的shRNA干扰表达载体pSi-p21;采用RT-PCR和Western blotting方法检测pSi-p21对p21 mRNA和蛋白表达的影响;通过DAPI核染色法检测pSi-p21抑制p21表达后姜黄素诱导Huh7细胞的凋亡情况。实验结果显示,姜黄素能明显上调p21 mRNA和蛋白的表达水平。干扰载体pSi-p21可以显著降低p21的表达水平和抑制姜黄素诱导肝癌细胞Huh7凋亡。研究结果提示,姜黄素通过上调p21的表达进而诱导肝癌细胞Huh7发生凋亡。In the present study, shRNA plasmid of pSi-p21 targeting p21 mRNA was constructed and the effect of p21 shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells was investigated. The effect of curcumin on the expression of p21 mRNA and protein and the silence efficiency of pSi-p21 were detected with RT-PCR and Westem blotting. The effect of pSi-p21 on curcumin-induced apoptosis of Huh7 cells was evaluated with DAPI staining. The results showed that curcumin significantly upregulated p21 mRNA and protein expression, which was knocked down by pSi-p21 of Huh7 cells. DAPI staining results showed that pSi-p21 significantly decreased curcumin-induced apoptosis of Huh7 cells. The data suggested that curcumin induced apoptosis of Huh7 cells via upregulation of p21 expression.

关 键 词：p21shRNA 姜黄素 肝癌细胞Huh7 细胞凋亡 

分 类 号：R963[医药卫生—微生物与生化药学] R979.1[医药卫生—药理学]