基于生物热活性检测的黏菌素效价测定方法研究  

Determination of colistin based on biothermal activity detection method

作　　者：罗云[1,2] 鄢丹[1] 任永申[1,2] 张少锋[1,3] 冯雪[1,4] 李寒冰[1,2] 唐慧英[1,3] 肖小河[1] 

机构地区：[1]解放军中药研究所解放军第三0二医院,北京100039 [2]都中医药大学药学院,四川成都610075 [3]江西中医学院,江西南昌330004 [4]首都医科大学,北京100069

出　　处：《药学学报》2009年第10期1136-1139,共4页Acta Pharmaceutica Sinica

基　　金：国家杰出青年科学基金项目(30625042);国家“十一·五”科技支撑计划重点项目(2006BAI08B03);国家自然科学基金资助项目(30873385)

摘　　要：采用生物热活性检测法,测定黏菌素作用于大肠杆菌的生物热活性谱,并以大肠杆菌生长时间延迟率Δt%为评价指标,建立黏菌素效价测定的新方法。结果显示,在17.0～41.6u·mL-1内,黏菌素浓度的对数值lgC与Δt%具有良好的线性关系(r=0.993);平均回收率为100.3%(n=9),且生物热活性检测法测定结果与管碟法测定结果一致(P>0.05)。结果表明,所建立的生物热活性检测法具有灵敏准确、实时在线、操作简便、干扰因素少、重现性好的特点,可作为黏菌素效价测定方法之一,同时所得生物热活性谱可弥补管碟法对细菌生长过程监控的不足。A biothermal activity detection method has been established to determine the potency of colistin.The biothermal activity fingerprints of E.coli with colistin were determined.There was a good linear relationship（r = 0.993） between logarithm concentration of colistin（lgC） and lag rate of growing time（Δ t %） when the concentrations of colistin ranged from 17.0 to 41.6 u·mL^-1.The average recovery rate was 100.3%（n = 9）.Using this method,there was no significant difference between results of colistin potency measurement and those using cup-plate method（P 〉 0.05）.As a result,biothermal activity detection method is sensitive,accurate,rapid,convenient and feasible to determine the potency of colistin.This method can also be applied in real time and online to monitor the process of bacterial growth and could be complementary to the cup-plate method.

关 键 词：生物热活性检测 生物检定 效价 抗生素 黏菌素 

分 类 号：Q939.9[生物学—微生物学]