新型原位聚合注射性人工髓核假体的遗传毒性研究  

A study of genetic toxicity of a neotype in situ polymeric injectable artificial prosthetic nucleus pulposus

作　　者：白克文[1] 邹德威[1] 吴继功[1] 程晓非[1] 周雪峰[1] 马华松[1] 

机构地区：[1]解放军第306医院骨科,北京100101

出　　处：《解放军医学杂志》2009年第10期1185-1187,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家高技术研究发展计划(863)资助项目(2006AA02Z4D4)

摘　　要：目的检测新型原位聚合注射性人工髓核材料的遗传毒性。方法制备新型人工髓核材料的浸提液,用于遗传毒性检测,包括鼠伤寒沙门菌回复突变试验(Ames试验)、利用中国仓鼠卵巢细胞(CHO)进行的哺乳动物细胞染色体畸变(CA)试验和小鼠微核(MN)试验,从对DNA的影响、染色体畸变和基因突变三种水平检测新型人工髓核材料的遗传毒性。结果Ames试验中受试样品各个剂量组中5个试验菌株在加入代谢活化系统(S9)和不加S9条件下,回变菌落数均低于阴性对照组的1/2,因此判定Ames试验结果为阴性。染色体畸变试验中,高、中、低三个浓度的试验组体外哺乳动物细胞染色体畸变率与阴性对照组比较无显著差异(P>0.05),但均显著低于阳性对照组(P<0.01)。微核试验中,试验组的微核率和微核细胞率与阴性对照组比较无显著差异(P>0.05),但均显著低于阳性对照组(P<0.01)。结论作为一种新型的体内内植物,原位聚合注射性人工髓核假体无明显遗传毒性。Objective To detect the genetic toxicity of a neotype in situ polymeric injectable artificial prosthetic nucleus pulposus.Methods The artificial prosthetic nucleus pulposus was soaked for preparing the leaching liquor which was used for tests of genetic toxicity.Salmonella typhimurium reverse mutation test（Ames Test）,mammalian cell chromosome aberration（CA） test utilizing Chinese hamster ovary cells（CHO）,and mouse micronucleus（MN） test were performed to detect the genetic toxicity of the extraction,including the effects on DNA,chromosome aberration and genetic mutation.Results The number of reverse mutation strains,from five strains of every dosage group,was all lower than half of quantum of control group,with or without the addition of S9,in the Ames Test,which assessed as negative.In CA test,no significant difference of chromosome aberration rate existed among the high,medium and low concentration group,while the CA rate of all the three groups was lower than that of the negative group（P〈0.01）.In MN test,micronucleus cell rate of the experiment group showed no significant difference compared with that of the negative control group（P〉0.05）,but significantly lower than that of the positive control group（P〈0.01）.Conclusion The in situ polymeric injectable artificial prosthetic nucleus pulposus,as a neotype of implants,shows no significant genetic toxicity.

关 键 词：生物相容性材料 关节成形术 人工髓核 诱变力试验 

分 类 号：R681.5[医药卫生—骨科学] R318.08[医药卫生—外科学]