大黄素对Jurkat细胞凋亡的诱导作用及其机制的初步研究  

Effect of Emodin on Induction of Apoptosis in Jurkat Cells and Its Possible Mechanisms

作　　者：魏天南[1] 胡建达[1] 陈英玉[1] 陈鑫基[1] 刘庭波[1] 吕联煌[1] 

机构地区：[1]福建医科大学附属协和医院福建省血液病研究所,福建福州350001

出　　处：《中国实验血液学杂志》2009年第5期1203-1206,共4页Journal of Experimental Hematology

基　　金：福建省高校新世纪优秀人才计划(编号NCETFJ-0604);福建医科大学教授基金(编号JS06081)

摘　　要：本研究探讨中药大黄素(emodin)对T淋巴细胞白血病Jurkat细胞株增殖、凋亡的影响及其作用机制。应用MTT法检测细胞增殖能力;DNA片段化检测和末端缺口原位标记(TUNEL)法分析细胞凋亡;蛋白印迹法(Western blot)检测BCL-2、C-MYC、hTERT、caspase蛋白前体procaspase-8、procaspase-9、procaspase-3和caspase-3剪切片段以及PARP等蛋白的表达水平。结果表明:大黄素能明显抑制Jurkat细胞增殖,对Jurkat细胞的半数抑制浓度(IC50)约为20μmol/L。DNA片段化的检出及TUNEL凋亡细胞的检出证实了大黄素能有效诱导Jurkat细胞凋亡,细胞凋亡率在一定的药物浓度范围内(0-80μmol/L)与药物作用浓度和作用时间呈正相关。Western blot检测结果显示,BCL-2、C-MYC和hTERT蛋白在大黄素作用后表达水平下降,caspase家族的蛋白前体procaspase-3、8、9表达均下降,而激活后的活性片段caspase-3则表达上调。结论:大黄素能有效抑制Jurkat细胞增殖,诱导其凋亡。其机制可能与下调BCL-2、C-MYC、hTERT等凋亡相关蛋白表达,激活caspase家族特别是caspase-3活性片段蛋白表达有关。The aim of study was to investigate the effect of a traditional Chinese medicine, emodin, on proliferation and apoptosis in T lymphocytic leukemic cell line Jurkat and its mechanisms. Cell proliferation inhibition was detected by MTT assay. Cell apoptosis was measured by DNA ladder and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling（TUNEL） assay. The expressions of related proteins and caspase family members were determined by Western blot. The results showed that emodin inhibited proliferation in Jurkat cells, with an IC50 about 20 μmol/L and induced cell apoptosis in both time-and dose-dependent manners. The expressions of proliferation-related protein C-MYC, hTERT and apoptosis-related protein BCL-2 were down-regulated in a time dependent manner after the treatment with emodin. The expressions of procaspase-3, -8 and -9 all decreased while activated casparse-3 and PARP expressions were up-regulated. It is concluded that emodin can remarkably inhibit cell proliferation and induce apoptosis in Jurkat cells. The down-regulation of proliferation-related proteins C-MYC, hTERT and apoptosis-related protein BCL- 2 expressions and activation of casnase cascade mav be involved in the process of apoptosis.

关 键 词：大黄素 凋亡 CASPASE途径 

分 类 号：R979.1[医药卫生—药品] R733.7[医药卫生—药学]