rhG-CSF对骨髓采集物中记忆T细胞上黏附分子表达的调节作用  

Modulation of Adhesion Molecule Expression on T Cells in Bone Marrow after In Vivo rhG-CSF Application

作　　者：霍明瑞[1] 常英军[1] 赵翔宇[1] 罗小华[1] 黄晓军[1] 

机构地区：[1]北京大学人民医院北京大学血液病研究所,北京100044

出　　处：《中国实验血液学杂志》2009年第5期1316-1320,共5页Journal of Experimental Hematology

基　　金：教育部创新团队发展计划基金(编号IRT0702);首都医学发展科研基金(编号2005-1010);国家杰出青年科学基金(编号30725038)

摘　　要：本研究主要探讨人重组粒细胞集落刺激因子(rhG-CSF)对骨髓采集物记忆T细胞上黏附分子表达的调节作用。采用流式细胞仪检测稳态骨髓(SS-BM)和rhG-CSF预激的骨髓(G-BM)中CD4+、CD8+T细胞百分比及其记忆细胞表面黏附分子CD49d、CD54、CD62L和CD11a的表达。结果显示:rhG-CSF应用后骨髓采集物中CD4+、CD8+T细胞在淋巴细胞中的比例明显降低(p<0.001),记忆T细胞的比例没有明显变化;CD49d在CD4+和CD8+T细胞的表达百分比显著下降(p<0.05),但在记忆T细胞的表达百分比没有明显的变化;CD54在CD4+及其记忆T淋巴细胞和CD8+T淋巴细胞的表达百分比明显降低(p<0.05),而在CD8+记忆T细胞的表达百分比没有明显变化;CD62L在CD4+、CD8+及其记忆T细胞的表达百分比显著下降(p<0.01);CD11a在CD4+及其记忆T细胞的表达百分比明显降低(p<0.05),而在CD8+及其记忆T细胞的表达百分比没有明显变化。结论:rhG-CSF部分下调骨髓中CD4+、CD8+以及相应的记忆T细胞上黏附分子表达。The aim of study was to investigate the modulation effect of recombinant human granulocyte colonystimulating factor （rhG-CSF） on adhesion molecule expression of memory T lymphocyte in the bone marrow grafts. rhG-CSF was administered in 41 donors by subcutaneous injection for 5 consecutive days. Bone marrow grafts were collected on day 4. The percentages of CD4^＋ and CD8 ^＋, and the expressions of CD49d, CD54, CD62L and CD1 la on donor T cells of steady state-bone marrow grafts （ SS-BM, n = 11 ） and rhG-CSF primed bone marrow （ G-BM, n = 30） were analyzed by using multi-color flow cytometry. The results indicated that the percentages of CD4^＋ and CD8^＋ T cells were significantly lower in G-BM than those in SS-BM （p 〈 0.05 ）. There were no significant differences in the percentages of memory CD4^＋ and CD8^＋ T cells between SS-BM and G-BM （p 〉 0.05 ）. The expressions of CD49d on CD4^＋ and CD8^＋T cells were significantly lower in G-BM than that in SS-BM （p 〈 0.05 ）. Compared with SS-BM, the expressions of CD54 on CD4^＋ , memory CD4^＋ T cells and CD8^＋ T cells were.significantly lower in G-BM （p 〈0, 05）. The expressions of CD62L on CD4^＋ and CD8^＋ T cells and memory T cells were all signifcantly lower in G-BM （p values were all less than 0.001 ）. The expressions of CDlla on CD4^＋ , memory CD4^＋ T cells were significantly lower in G-BM than that in SS-BM （p 〈0.05）. There were no significant differences in the expression of CDlla on CD8^＋ , memory CD8^＋ T cells between SS-BM and G-BM （p 〉 0.05 ）. It is concluded that the expression of cell adhesion molecules on the CD4^＋ and CD8^＋ T cells in G-BM is down-regulated after rhG-CSF treatment of healthy donors.

关 键 词：黏附分子 稳态骨髓 骨髓移植物 记忆T细胞 粒细胞集落刺激因子 

分 类 号：R457.7[医药卫生—治疗学] R392.4[医药卫生—临床医学]