Identifying neutral allele Sb at pollen-sterility loci in cultivated rice with Oryza rufipogon origin  

作　　者：SHI LeiGang LIU XiangDong LIU Bo ZHAO XingJuan WANG Lan LI JinQuan LU YongGen 

机构地区：[1]Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China

出　　处：《Chinese Science Bulletin》2009年第20期3813-3821,共9页

基　　金：Supported by the Joint Funds of the National Natural Science Foundation of China;Guangdong Province(Grant No.U0631003);Natural Science Foundation of Guangdong Province(Grant No.5300831)

摘　　要：Pollen sterility is commonly found in the intra-specific hybrids of indica and japonica rice,which is one of the main constrains for the utilization of heterosis between indica and japonica.Six loci controlling the pollen sterility of F1 between indica and japonica have been identified from previous studies.Neutral alleles at each locus are potential to overcome the F1 pollen sterility associated with the locus.Therefore,exploitation and utilization of neutral alleles are of significant importance.The present research was based on fine mapping of the F1 pollen-sterility gene Sb and the abundant genetic diversity of Oryza rufipogon Griff.indigenous to Gaozhou,Guangdong Province(referred to as Gaozhou wild rice).Crosses were made using Taichung65(with the genotype of SbjSbj and referred to as E1) and its near-isogenic line of F1 pollen sterility gene Sb(with the genotype of SbiSbi,E2) as female parents,and 12 different accessions of Gaozhou wild rice as male parents.F1 pollen fertility was examined to identify the materials having the neutral alleles at the F1 pollen-sterility locus.Segregation of 4 molecular markers tightly linked with the Sb locus was analyzed in the F2 populations derived from the F1s carrying the neutral gene.The pollen fertility related to the 3 genotypes of the molecular markers was also checked by statistical test to determine whether it was consistent with the hypothesis.The results showed that the pollen fertility of two F1s from one accession of Gaozhou wild rice(GZW099) with E1 and E2 was(89.22±1.07)% and(85.65±1.05)%,respectively.Both of them were fertile and showed no significant difference by t-test.Segregation of the 3 genotypes of the 4 molecular markers followed the expected Mendelian ratio(1:2:1) in the F2 populations.There was no significant difference for the averaged pollen fertility of the plants related to the 3 genotypes,suggesting that no interaction exists between the alleles at the Sb locus in GZW099 and Taichung65 or E2.Evidentially,GZW099 carried the neutral gene(namePollen sterility is commonly found in the intra-specific hybrids of indica and japonica rice, which is one of the main constrains for the utilization of heterosis between indica and japonica. Six loci controlling the pollen sterility of F1 between indica and japonica have been identified from previous studies. Neutral alleles at each locus are potential to overcome the F1 pollen sterility associated with the locus. Therefore, exploitation and utilization of neutral alleles are of significant importance. The present research was based on fine mapping of the F1 pollen-sterility gene Sb and the abundant genetic diversity of Oryza rufipogon Griff. indigenous to Gaozhou, Guangdong Province （referred to as Gaozhou wild rice）. Crosses were made using Taichung65 （with the genotype of Sb^jSb^j and referred to as El） and its near-isogenic line of F1 pollen sterility gene Sb（with the genotype of Sb^iSb^i, E2） as female parents, and 12 different accessions of Gaozhou wild rice as male parents. F1 pollen fertility was examined to identify the materials having the neutral alleles at the F1 pollen-sterility locus. Segregation of 4 molecular markers tightly linked with the Sb locus was analyzed in the F2 populations derived from the FlS carrying the neutral gene. The pollen fertility related to the 3 genotypes of the molecular markers was also checked by statistical test to determine whether it was consistent with the hypothesis. The results showed that the pollen fertility of two F1s from one accession of Gaozhou wild rice （GZW099） with E1 and E2 was （89.2±21.07）% and （85.65±1.05）%, respectively. Both of them were fertile and showed no significant difference by t-test. Segregation of the 3 genotypes of the 4 molecular markers followed the expected Mendelian ratio （1：2：1） in the F2 populations. There was no significant difference for the averaged pollen fertility of the plants related to the 3 genotypes, suggesting that no interaction exists between the alleles at the Sb locus in GZW099 and Taic

关 键 词：遗传学 等位基因 稻子 杂交 

分 类 号：S511[农业科学—作物学]