Apoptosis induced by titanium dioxide nanoparticles in cultured murine microglia N9 cells  

作　　者：LI XiaoBo XU ShunQing ZHANG ZhiRen Hermann J Schluesener 

机构地区：[1]School of Public Health, Southeast University, Nanjing 210009, China [2]Key Laboratory of Environment and Heath of the Ministry of Education, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [3]Institute of Brain Research, University of Tuebingen, Calwer Str. 3, D-72076 Tuebingen, Germany

出　　处：《Chinese Science Bulletin》2009年第20期3830-3836,共7页

基　　金：Supported by the German Academic Exchange Service (DAAD) and Chinese Scholarship Council (CSC)

摘　　要：Owing to the rapidly increasing output of nano-scale titanium dioxide(TiO2) particles,their potential risk for central nerve system(CNS) has elicited much concern recently.Microglia is the resident macrophage in CNS and essential for the homeostasis of the CNS microenvironment.They are supposed to response to nanoparticles depositing in the brain tissues.Therefore,we investigated the cytotoxic effects of TiO2 NPs on microglia N9 cells in vitro.Results of propidium iodide/fluorescein diacetate(PI/FDA) double staining and MTT test clearly showed that TiO2 NPs more efficiently affected the viability of microglia N9 cells.Further Hoechst 33258 staining and flow cytometric analysis proved that nano-scale but not normal scale TiO2 induced apoptosis in vitro.These data suggest that TiO2 NPs can elicit apoptosis of N9 cells in vitro and thus present a potential risk for CNS.Owing to the rapidly increasing output of nano-scale titanium dioxide （TiO2） particles, their potential risk for central nerve system （CNS） has elicited much concern recently. Microglia is the resident macrophage in CNS and essential for the homeostasis of the CNS microenvironment. They are supposed to response to nanoparticles depositing in the brain tissues. Therefore, we investigated the cytotoxic effects of TiO2 NPs on microglia N9 cells in vitro. Results of propidium iodide/fluorescein diacetate （PI/FDA） double staining and MTT test clearly showed that TiO2 NPs more efficiently affected the viability of microglia N9 cells. Further Hoechst 33258 staining and flow cytometric analysis proved that nano-scale but not normal scale TiO2 induced apoptosis in vitro, These data suggest that TiO2 NPs can elicit apoptosis of N9 cells in vitro and thus present a potential risk for CNS.

关 键 词：纳米二氧化钛 胶质细胞 细胞凋亡 诱导培养 中枢神经系统 二氧化钛纳米粒 流式细胞仪分析 小鼠 

分 类 号：Q421[生物学—神经生物学] TQ134.11[生物学—生理学]