模拟微重力环境下细菌生物学效应的初步研究  

Study on Biological Effects of Simulated Microgravity on Bacteria

作　　者：尹焕才[1,2] 薛小平[1] 杨慧[1] 谢玉为[1] 唐蕊华[1] 苏婧[1] 宋凯[1] 呼延霆[1] 汪桦[1] 

机构地区：[1]西北工业大学生命科学学院,陕西西安710072 [2]中国科学院苏州生物医学工程技术研究所,江苏苏州215163

出　　处：《航天医学与医学工程》2009年第5期341-346,共6页Space Medicine & Medical Engineering

摘　　要：目的研究模拟微重力环境下细菌的生物学效应。方法应用回转器处理实验菌株(大肠杆菌、金黄色葡萄球菌、枯草芽胞杆菌、鼠伤寒沙门氏菌),检测其生长曲线,抗生素敏感性,以及抗生素敏感性变化株与野生株间脂肪酸图谱的差别。结果模拟微重力条件下,细菌对数期提前、生长曲线形状改变。回转器处理30d后可检测到大肠杆菌抗生素敏感性突变株,并且突变株的数量与处理时间呈现正相关趋势;鼠伤寒沙门氏菌处理120d后,检测到氨苄西林和左氧沙星耐受株突变为敏感株;脂肪酸图谱分析显示,突变株与对照株相比脂肪酸的种类和含量存在一定差异。结论回转器模拟微重力条件下,细菌生物性状受到影响,抗生素敏感性的改变可能与细菌脂肪酸图谱的变化有关。Objective To investigate biological effects of simulated microgravity on bacteria. Methods Four strains of bacteria,Escherichia coli,Staphylococcus aureus,Bacillus subtilis,Salmonella typhimurium were pre-treated under simulated microgravity with clinostat. We compared biochemical characters of treated groups with control groups respectively,including growth curves,sensitivity to antibiotics and the differences of fatty acid composition spectra between the sensitivity to antibiotics of mutant strains and wild strains. Results The profiling behavior of the treated groups showed great distinction from the control ones. The lag phase advanced, and the growth curves turned to a different shape. After continuous cultivation with clinostat for 30 d, the sensitive mutant strains to antibiotics of E. coli emerged, and the number of sensitive strains increased by time. After 120 d treated cultivation, ampicillin and levofloxacin resistant Salmonella typhimurium strains mutated to sensitive strains. Compared the spectra of fatty acids of the mutation strains with control, it was found that the compositions and relative content of fatty acids changed. Conclusion The sensitivity to antibiotics of these bacteria may change under simulated microgravity. The variation of composition of fatty acids of these bacteria implies that the composition of fatty acids may be a factor which affects the sensitivity to antibiotics of bacteria

关 键 词：模拟微重力 回转器 抗生素敏感性 脂肪酸 

分 类 号：Q693[生物学—生物物理学] R851[医药卫生—航空、航天与航海医学]