近日节律基因Per2对人肺癌细胞生长的抑制作用  

Inhibitory Effects of Circadian Gene Per2 on Human Pulmonary Carcinoma Cell

作　　者：芮军[1] 华慧[2] 刘延友[2] 

机构地区：[1]成都市第三人民医院胸外科,四川成都610031 [2]四川大学卫生部时间生物学重点实验室,四川成都610041

出　　处：《航天医学与医学工程》2009年第5期378-380,共3页Space Medicine & Medical Engineering

摘　　要：目的研究近日节律基因Per2对肺癌细胞(A549)生长的抑制作用及其机制。方法将pcDNA3.1(+)-Per2转导入A549细胞内,过表达Per2,以空质粒pcDNA3.1(+)为对照。采用MTT和细胞平板集落形成实验检测肺癌细胞的生长情况;通过流式细胞技术观察细胞的凋亡。结果近日节律基因Per2能够抑制A549细胞的生长和增殖,诱导细胞凋亡。结论近日节律基因Per2能够抑制肺癌细胞的生长,其机制可能涉及诱导肿瘤细胞的凋亡。Objective To reveal the effects of circadian gene Per2 on growth of human lung cancer cell A549 cell and its mechanism. Methods pcDNA3.1（＋）-Per2 was transfected into A549 cells and controlled with the vector pcDNA3.1（＋）. The expression of Per2 was confirmed with RT-PCR and western blot. The Inhibitory effect of Per2 on cell growth and proliferation was demonstrated with MTT assay and colony-forming assay. Apoptosis was detected with flow cytometry. Results The circadian gene Per2 exhibited a growth-inhibitory and apoptosis-inductory effect on A549 cells. Conclusion The results indicate that Per2 may inhibit A549 cell proliferation, which mechanism might involve cellular apoptosis.

关 键 词：近日节律基因 肿瘤 凋亡 肺癌 

分 类 号：R852.6[医药卫生—航空、航天与航海医学] Q789[医药卫生—临床医学]