中国李pgip启动子的克隆及调控元件分析  

Cloning of pgip Promoter from Prunus salicina and the Computational Identification of Its Regulatory Element

作　　者：李广平[1] 张长青[2] 章镇[3] 曹福亮[1] 

机构地区：[1]南京林业大学森林资源与环境学院,南京210037 [2]金陵科技学院园艺系,南京210038 [3]南京农业大学园艺学院,南京210095

出　　处：《园艺学报》2009年第10期1425-1430,共6页Acta Horticulturae Sinica

基　　金：南京林业大学高学历人才基金项目(163010065)

摘　　要：在已克隆中国李pgip序列的基础上,通过染色体步行法获得了该基因上游1869bp的启动子序列,联合生物信息学中的进化印记法和启动子扫描法对克隆的启动子序列进行了调控元件识别。结果报道了3个调控元件:1个TGA1结合位点(TGACG)和2个WRKY结合位点(TGAC)。这为全面揭示pgip表达的转录调控机制提供了遗传基础。Based on the gene sequence of pgip, we have obtained an upstream fragment of 1 869 bp length by genome walker from Prunus salicina. Using the bioinformatics methods, including the phylogenetic footprinting and the promoter scanning, we identified three cis-regulatory elements from pgip promoter. One is the TGA1 site and the others are WRKY sites. Our discoveries will be help to understand the transcriptional regulatory mechanism on pgip expression.

关 键 词：中国李 pgip启动子 克隆 调控元件分析 

分 类 号：S662.3[农业科学—果树学]