番木瓜eIF(iso)4E基因克隆及其结构和表达分析  

eIF(iso)4E Full Sequence Cloning and Structure and Expression Analyzing in Papaya

作　　者：言普[1] 沈文涛[1] 高新征[1] 李亚丽[1] 周鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点实验室,海口571101

出　　处：《园艺学报》2009年第10期1437-1442,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30760134);中央级科研院所基本科研业务费项目(ITBBZD0732);海南省自然科学基金项目(808186)

摘　　要：eIF4E(eukaryotic translation initiation factor4E)家族基因在多种病毒侵染植物过程中起重要作用。应用RT-PCR和RACE方法在番木瓜中克隆了cDNA全长为995bp的eIF4E异构体基因,命名为CP-eIF(iso)4E(GenBank登录号为FJ595992)。其编码的蛋白含有206个氨基酸,蛋白一级结构中的活性部位位点高度保守,三维结构中可能的抗性位点位于帽结合区域(cap-binding pocket)表面。半定量RT-PCR结果表明,CP-eIF(iso)4E在番木瓜叶片中表达较弱,在花瓣中表达最强。eIF4E （eukaryotic translation ruination tactor 4E） famliy genes pray important roles against virus from infecting plants. A complete cDNA of 995 bp from papaya was cloned using the method of RT-PCR and RACE. It was a isoform eIF4E, so it was named CP-elF（iso）4E （GenBank accession No. FJ595992）. The predicted CP-eIF （iso） 4E protein had 206 amino acids, in which the active site was high conservative in primary structure, and the predicted virus-resistance site was located on the surface of cap-binding pocket in 3-D structure. Semi-quantitative RT-PCR analysis indicated that CP-eIF （iso） 4E expression was lower in leaf and higher in petal of papaya.

关 键 词：番木瓜 eIF(iso)4E 结构分析 基因表达 

分 类 号：S667.9[农业科学—果树学] Q78[农业科学—园艺学]