西双版纳黄瓜群体遗传多样性的SSR分析  

Genetic Diversity Analyses of Cucumis sativus L. var. xishuangbannanesis Population Based on SSR Markers

作　　者：沈镝[1] 方智远[1] 戚春章[1] 赵金艳[1] 宋江萍[1] 李全辉[1] 李锡香[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2009年第10期1457-1464,共8页Acta Horticulturae Sinica

基　　金：国家科技支撑计划项目(2006BAD13B06-3-4);科技部科技基础性工作专项课题(2006FY110708);农业部园艺作物遗传改良重点开放实验室项目

摘　　要：西双版纳黄瓜（Cucumis sativus L.var. xishuangbannanesis Qi et Yuan）是我国特有的黄瓜变种。以20世纪70年代末收集的30份西双版纳黄瓜种质273个样本为研究对象,利用SSR技术从群体水平分析其遗传多样性。273个样本的多态位点百分率、Nei s基因多样性指数（H）和Shannon信息指数（I）分别为92.86%、0.1818和0.2972。比较30份种质的遗传多样性指标,显示种质间遗传变异54.06%,种质内遗传变异45.94%。17号种质的遗传多样性最高（H=0.1376,I=0.2032,多态位点百分率37.21%）。聚类分析结果显示,大多数种质的分组与瓜皮色和瓜肉色基本一致。按不同来源地将种质分为5个群体,其中景洪群体的遗传多样性最高,其多态位点百分率、H值和I值分别为76.19%、0.1562和0.2516。群体间遗传一致度在0.9071～0.9889之间。聚类分析结果显示不同来源地的西双版纳黄瓜在遗传组成上存在差异。群体遗传分化系数和基因流分别为0.2201和1.7718,表明西双版纳黄瓜群体间基因分化不明显,基因交流基本顺畅。Xishuangbanna cucumber （Cucumis sativus L. vat. xishuangbannanesis Qi et Yuan） is a special variation in our country. Simple sequence repeat （SSR） analyses were conducted to assess the genetic diversity of 30 germplasm resources collected in Xishuangbanna prefecture in 1979 and 1980, which showed that relatively abundant genetic diversity existed in Xishuangbanna cucumber population. The percentage of polymorphic loci, Nei＇s gene diversity （H） and Shannon information index （I） of 273 samples were 92. 86% , 0. 1818 and 0. 2972, respectively. The indexes of genetic diversity of 30 germplasm showed that the genetic variation among germplasm was 54.06% and that within germplasm was 45.94%. The genetic diversity of No. 17 germplasm was the highest among 30 germplasm （H = 0. 1376, I = 0. 2032, percentage of polymorphic loci = 37.21% ）. Cluster analysis showed that the result of classification of most germplasm was consistent with fruit skin color and flesh color. Among 5 populations according to accession source, the genetic diversity of Jinghong population was the highest, whose polymorphic loci, H value and 1 value were 76. 19%, 0. 1562 and 0. 2516 respectively. Genetic identity of 5 populations was between 0. 9071 and 0. 9889. Cluster analyses suggest that the difference of genetic background existed in 5 populations with different collecting source. The coefficient of genetic differentiation （Gst） and gene flow （Nm） were 0. 2201 and 1. 7718, which indicated that the genetic differentiation was relatively low, with relatively larger gene flow in C. sativus L var. xishuaugbannanesis.

关 键 词：黄瓜 西双版纳黄瓜 SSR 遗传多样性 群体 

分 类 号：S642.2[农业科学—蔬菜学]